This protocol is significant because it quantitatively measures phagocytosis of labeled Aspergillus fumigateurs Conidia by human leukocytes, along with the attachment of Conidia to cells, and lack of interaction by flow cytometry. The main advanta
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This protocol provides a fast and reliable method to quantitatively measure phagocytosis of Aspergillus fumigatus conidia by human primary phagocytes using flow cytometry and to discriminate phagocytosis of conidia from mere adhesion to leukocytes.