Leibniz Institute for Infection Biology and Natural Product Research

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

Susann Hartung^1,2, Christopher Rauh², Sarah Böttcher¹, Mai Thi Ngoc Hoang^1,2, Susanne Jahreis^1,2, Silke Rummler³, Andreas Hochhaus², Marie von Lilienfeld-Toal^1,2

¹Infections in Hematology and Oncology, Leibniz Institute for Infection Biology and Natural Product Research, ²Department for Hematology and Medical Oncology, Jena University Hospital, ³Institute for Transfusion Medicine, Jena University Hospital

This protocol provides a fast and reliable method to quantitatively measure phagocytosis of Aspergillus fumigatus conidia by human primary phagocytes using flow cytometry and to discriminate phagocytosis of conidia from mere adhesion to leukocytes.