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DOI :
10.3791/60398-v
November 6th, 2019
Chapters
0:04
Title
1:48
Sample Preparation
2:38
TIRF Imaging: Alignment of the Laser Line and Optimization of TIRF Illumination
4:05
TIRF Imaging: Capture of the Time Series
5:46
Number & Brightness (N&B): Quality Check of the Time Series
6:34
Number & Brightness (N&B): Computation of the B-values in Selected Region-of-interest (ROI)
8:19
Results: N&B Analysis and Kinetics of FGFR1 Oligomerization
9:42
Conclusion
生細胞の形質膜におけるリガンド結合によって誘導されるmEGFPタグ付き受容体オリゴマーの平均オリゴマー状態の測定のためのイメージングアプローチについて説明する。このプロトコルは、数と明るさ(N&B)分析と組み合わせた全内部反射蛍光(TIRF)顕微鏡に基づいています。
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