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DOI :
10.3791/60400-v
•
8:49 min
March 18th, 2020
Chapters
0:04
Introduction
1:09
Stimulating Rhesus PBMCs with Plate-bound Anti-CD3 and Soluble Anti-CD28
2:47
Transduction of Activated Rhesus PBMCs
5:16
Expansion of the Transduced Cells
6:45
Results: Analysis of Transduced and Expanded Cells
8:01
Conclusion
我们概述了一个为期9天的协议,用于转导和扩大流黄猴外周外血单核细胞,该细胞产生具有良好共同表达的细胞,其感兴趣的基因数量足以用于细胞有效性的输液研究。
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