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DOI :
10.3791/60400-v
•
8:49 min
March 18th, 2020
Chapters
0:04
Introduction
1:09
Stimulating Rhesus PBMCs with Plate-bound Anti-CD3 and Soluble Anti-CD28
2:47
Transduction of Activated Rhesus PBMCs
5:16
Expansion of the Transduced Cells
6:45
Results: Analysis of Transduced and Expanded Cells
8:01
Conclusion
우리는 세포 효능의 주입 연구를 위한 충분한 수에 있는 관심 있는 유전자의 우수한 공동 발현을 가진 세포를 산출하는 rhesus macaque 말초 혈액 단핵 세포의 transduction 그리고 확장을 위한 9 일 프로토콜을 개략적으로 설명합니다.
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