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DOI :
10.3791/60406-v
•
7:44 min
November 28th, 2019
Chapters
0:05
Title
1:09
Day 1: Inoculation and Acclimation of Cultures
1:49
Day 2: Generation of Mutants in Parallel Cultures
3:27
Day 3: Plating Cultures on Selective and Non-selective Agar Plates
4:48
Day 4 and Day 5
5:48
Results: Analysis of the Fluctuation Assay
6:47
Conclusion
ここで、フェノールトマーカーを用いて変動アッセイを行い、微生物変異率を推定するプロトコルが提示される。このプロトコルにより、研究者は多様な微生物や環境における突然変異をアッセイし、遺伝子型や生態学的文脈が自発的な突然変異率に与える影響を判断することができます。
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