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JoVE Journal

Bioengineering

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Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

6.9K Views

10:53 min

October 13th, 2019

October 13th, 2019

6,918 Views

0:04

Title

0:47

Fabrication of Polydimethylsiloxane (PDMS) Stencil and Microchannel

2:39

Preparation of Bottom Glass with Polyacrylamide (PA) Gel

4:41

Micropatterning of Cell Islands and Assembly of PA Gel with PDMS Microchannel

6:14

Integrated Microfluidic System

7:32

Image Acquisition

8:27

Results: Mechanical Interactions of MDCK Cell Islands

9:55

Conclusion

Transcript

Collective cell migration is often guided by the gradient of signaling molecules. In this video, we demonstrate how to quantify cellular forces during collective migration guided by biochemical gradient. So, we integrate the microfluidic chip with

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Collective cell migration in development, wound healing, and cancer metastasis is often guided by the gradients of growth factors or signaling molecules. Described here is an experimental system combining traction microscopy with a microfluidic system and a demonstration of how to quantify the mechanics of collective migration under biochemical gradient.

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