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13.0K Views
•
07:23 min
December 3rd, 2019
DOI :
10.3791/60419-v
Chapters
0:05
Title
1:12
Preparation of P. aeruginosa PAO1 Culture
1:58
Preparation of Nematode Growth Medium (NGM) Plates for Testing the Effects of Bacteria and DIM on the Intestinal Permeability of C. elegans
2:57
Bacteria or DIM Treatment and FITC-Dextran Feeding
4:19
Imaging of C. elegans with the Operetta Imaging System and Determination of Intestinal Permeability
5:43
Results: Effects of Bacteria on Intestinal Permeability of P. aeruginosa
6:40
Conclusion
Transcript
该方法将有助于与肠道微生物群与肠道健康相互作用相关的基本生物学研究。此外,此协议可用于筛选潜在的益生菌和营养素,以治疗肠道健康问题,如肠道漏位综合征和炎症性肠道疾病。该协议使用高通量成像系统,能够快速、精确地定量分析使用各种细菌和化学品处理的C.elegans肠道渗透性。
我们建议不熟悉此技术的科学家限制样品数量,以避免错误标记和其他错误。此过程包括许多需要可视化演示的步骤,例如将蠕虫转移到 96 孔板和使用 Operetta 机器以及和谐软件。首先准备500毫升无菌LB介质。
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Summary
该协议描述了如何测量卡内沙布迪炎的肠道渗透性。该方法有助于对肠道健康进行与肠道细菌与其宿主相互作用相关的基础生物学研究,并有助于筛选出益生菌和化学制剂,以治疗漏肠综合征和炎症性肠病。
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