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DOI :
10.3791/60427-v
•
8:19 min
June 22nd, 2020
Chapters
0:04
Introduction
1:00
Skinned Cardiomyocyte Extraction and Permeabilization
2:46
Skinned Cardiomyocyte Selection and Gluing
4:03
Active, Passive and Calcium (Ca2+) Sensitivity Force Measurements
5:58
Results: Representative Skinned Cardiomyocyte Assessment
7:36
Conclusion
このプロトコルは、皮をむいた心筋細胞を用いた心機能の抽出および評価の技術を段階的に記述することを目的とする。この方法論は、マウスから男性まで、異なる心臓の場所から収集することができる小さな凍結生検を使用して、myofilament機能の測定および急性調節を可能にする。
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