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DOI :
10.3791/60469-v
•
8:43 min
January 31st, 2020
Chapters
0:04
Title
0:50
In Vitro Culture of Bladder Tumor Organoids
3:50
Genetic Manipulation of Bladder Organoids via Lentivirus-mediated Transduction
4:36
Orthotopic Transplantation of Bladder Organoids
7:06
Results: Culture and Transplantation of Bladder Tumor Organoids
8:01
Conclusion
このプロトコルは、発癌性癌誘発性マウス膀胱癌に由来する膀胱腫瘍オルガノイドの三次元体内培養を確立するための詳細な実験ステップを提供する。腫瘍オルガノイドの受動法、遺伝子工学、および正体移植を含む培養方法が記載されている。
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