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DOI :
10.3791/60485-v
•
6:28 min
February 13th, 2020
Chapters
0:05
Introduction
0:53
Human Subcutaneous Adipose Tissue Dissection
1:32
Collagenase Treatment
2:21
Mature Adipocyte Purification
3:20
Mature Adipocyte Packing
3:51
Mature Adipocyte Seeding and Maintenance
4:50
Results: Representative Mature Adipocyte Aggregate Culture (MAAC) Appearance and Response to Diverse Pharmacological Agonism
5:55
Conclusion
膜成熟脂肪细胞聚集培养(MAAC)是培养成熟人类脂肪细胞的新方法。在这里,我们详细介绍了如何从人类脂肪中分离脂肪细胞,以及如何设置MAAC。
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