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JoVE Journal

Bioengineering

This content is Open Access.

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Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

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08:22 min

February 23rd, 2020

February 23rd, 2020

9,280 Views

0:04

Title

1:12

Loading and Operation of the EWOD Chip on the DMF Platform

4:10

Operating in Visual Mode

5:03

Removing Liquid Waste and Cleaning the Chip

5:48

Results: Optimization of Immunoassays on the EWOD Chip

7:24

Conclusion

Transcript

Electrowetting-based digital microfluidic is a technique that utilizes a voltage-driven change in the apparent contact angle of a microliter-volume droplet to facilitate its manipulation. Combining this with functionalized magnetic beads enables the integration of multiple laboratory unit operations for sample preparation and identification of pathogens using Enzyme-linked Immunosorbent Assay (ELISA).

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