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DOI :
10.3791/60490-v
•
12:52 min
March 5th, 2020
Chapters
0:05
Introduction
1:33
Cell Isolation
2:57
Cardiomyocyte Culture
4:17
Patch-Clamp Experiment
5:44
Carbon Fiber Preparation
7:40
Cardiomyocyte Contraction Force Recording
10:23
Results: Representative Patch-Clamp and Carbon Fiber Recordings
11:44
Conclusion
우리는 토끼 심근 세포에서 GtACR1 활성화의 전기 기계적 효과를 평가하기위한 프로토콜을 제시한다. 당사는 세포 격리, 배양 및 아데노바이러스 전이, 패치 클램프 및 탄소 섬유 기술로 기능적 실험에 대한 자세한 정보를 제공합니다.
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