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Whole-Mount In Situ Hybridization i Zebrafish Embryoner og Tube Formation Assay i iPSC-ECs at studere den rolle, Endoglin i vaskulær udvikling

DOI :

10.3791/60498-v

May 28th, 2020

May 28th, 2020

7,491 Views

1Department of Physiology and Department of Cardiology of the Second Affiliated Hospital, Zhejiang University School of Medicine, 2Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 3Wuxi Lung Transplant Center, Wuxi People's Hospital affiliated to Nanjing Medical University

Præsenteret her er en protokol for hele mount in situ RNA hybridisering analyse i zebrafisk og rør dannelse assay i patient-afledt induceret pluripotente stamceller-afledte endotelceller til at studere den rolle, endoglin i vaskulær dannelse.

Tags

Udviklingsbiologi

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