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Kvantificering af proliferative og døde celler i Enteroids

DOI :

10.3791/60501-v

January 31st, 2020

January 31st, 2020

8,131 Views

1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, 2Department of Pathology, University of Chicago, 3Department of Pathology, Brigham and Women's Hospital–Harvard Medical School

Den præsenterede protokol bruger flow cytometri til at kvantificere antallet af prolifererende og døde celler i dyrkede mus enteroids. Denne metode er nyttig til at vurdere virkningerne af narkotikabehandling på organoid spredning og overlevelse.

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Biologi

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