Name: coronary progenitor cells and soluble biomarkers in cardiovascular prognosis after coronary angioplasty
Uploaded: 2020-01-28T13:00:00
Description: Watch this Scientific Journal Video about Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty at JoVE.com

作者感谢机构计划E015的支持;和Fondo部门FOSSIS-CONACYT，SALUD-2014-1-233947。

该协议符合人类研究伦理委员会的体制准则。
1. 冠状血管造影、超声和血液取样
<ol><li>在冠状动脉介入之前，请求基线临床和人口统计信息。收集个人数据：年龄、性别、当前吸烟状态、体重指数（BMI）、高血压、血脂异常、糖尿病、药物和当前冠状血管造影指标。</li>
	<li>使用径向方法通过心脏导管进行冠状血管造影。此程序应在由专家心脏病学家在血管动力学室的荧光?...

<ol>
	<li>Cassar, A., Holmes, D. R., Rihal, C. S., Gersh, B. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+coronary+artery+disease:+diagnosis+and+management.">Chronic coronary artery disease: diagnosis and management.</a> Mayo Clinic Proceedings. 84 (12), 1130-1146 (2009).</li><li>Regueiro, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mobilization+of+endothelial+progenitor+cells+in+acute+cardiovascular+events+in+the+PROCELL+study:+time-course+after+acute+myocardial+infarction+and+stroke.">Mobilization of endothelial progenitor cells in acute cardiovascular events in the PROCELL study: time-course after acute myocardial infarction and stroke.</a> Journal of Molecular and Cellular Cardiology. 80, 146-155 (2015).</li><li>Sen, S., McDonald, S. P., Coates, P. T., Bonder, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelial+progenitor+cells:+novel+biomarker+and+promising+cell+therapy+for+cardiovascular+disease.">Endothelial progenitor cells: novel biomarker and promising cell therapy for cardiovascular disease.</a> Clinical Science (Lond). 120 (7), 263-283 (2011).</li><li>Samman Tahhan, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Progenitor+Cells+and+Clinical+Outcomes+in+Patients+With+Acute+Coronary+Syndromes.">Progenitor Cells and Clinical Outcomes in Patients With Acute Coronary Syndromes.</a> Circulation Research. 122 (11), 1565-1575 (2018).</li><li>Tomuli&#263;, V., Gobi&#263;, D., Luli&#263;, D., &#381;idan, D., Zaputovi&#263;, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Soluble+adhesion+molecules+in+patients+with+acute+coronary+syndrome+after+percutaneous+coronary+intervention+with+drug-coated+balloon,+drug-eluting+stent+or+bare+metal+stent.">Soluble adhesion molecules in patients with acute coronary syndrome after percutaneous coronary intervention with drug-coated balloon, drug-eluting stent or bare metal stent.</a> Medical Hypotheses. 95, 20-23 (2016).</li><li>Jaumdally, R., Varma, C., Macfadyen, R. J., Lip, G. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Coronary+sinus+blood+sampling:+an+insight+into+local+cardiac+pathophysiology+and+treatment?.">Coronary sinus blood sampling: an insight into local cardiac pathophysiology and treatment?.</a> European Heart Journal. 28 (8), 929-940 (2007).</li><li>Kremastinos, D. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracoronary+cyclic-GMP+and+cyclic-AMP+during+percutaneous+transluminal+coronary+angioplasty.">Intracoronary cyclic-GMP and cyclic-AMP during percutaneous transluminal coronary angioplasty.</a> International Journal of Cardiology. 53 (3), 227-232 (1996).</li><li>Karube, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measurement+of+cytokine+levels+by+coronary+sinus+blood+sampling+during+cardiac+surgery+with+cardiopulmonary+bypass.">Measurement of cytokine levels by coronary sinus blood sampling during cardiac surgery with cardiopulmonary bypass.</a> American Society for Artificial Internal Organs Journal. 42 (5), M787-M791 (1996).</li><li>Truong, Q. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Coronary+sinus+biomarker+sampling+compared+to+peripheral+venous+blood+for+predicting+outcomes+in+patients+with+severe+heart+failure+undergoing+cardiac+resynchronization+therapy:+the+BIOCRT+study.">Coronary sinus biomarker sampling compared to peripheral venous blood for predicting outcomes in patients with severe heart failure undergoing cardiac resynchronization therapy: the BIOCRT study.</a> Heart Rhythm. 11 (12), 2167-2175 (2014).</li><li>Su&#225;rez-Cuenca, J. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Coronary+circulating+mononuclear+progenitor+cells+and+soluble+biomarkers+in+the+cardiovascular+prognosis+after+coronary+angioplasty.">Coronary circulating mononuclear progenitor cells and soluble biomarkers in the cardiovascular prognosis after coronary angioplasty.</a> Journal of Cellular and Molecular Medicine. 23 (7), 4844-4849 (2019).</li><li>Su&#225;rez-Cuenca, J. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relation+of+Coronary+Artery+Lumen+with+Baseline,+Post-angioplasty+Coronary+Circulating+Pro-Inflammatory+Cytokines+in+Patients+with+Coronary+Artery+Disease.">Relation of Coronary Artery Lumen with Baseline, Post-angioplasty Coronary Circulating Pro-Inflammatory Cytokines in Patients with Coronary Artery Disease.</a> Angiology Open Access. 7, 01 (2019).</li><li>Schmidt-Lucke, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantification+of+circulating+endothelial+progenitor+cells+using+the+modified+ISHAGE+protocol.">Quantification of circulating endothelial progenitor cells using the modified ISHAGE protocol.</a> PLoS One. 5 (1), e13790 (2010).</li><li>Moyer, C. F., Sajuthi, D., Tulli, H., Williams, J. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synthesis+of+IL-1+alpha+and+IL-1+beta+by+arterial+cells+in+atherosclerosis.">Synthesis of IL-1 alpha and IL-1 beta by arterial cells in atherosclerosis.</a> American Journal of Pathology. 138 (4), 951-960 (1991).</li><li>Morales-Portano, J. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Echocardiographic+measurements+of+epicardial+adipose+tissue+and+comparative+ability+to+predict+adverse+cardiovascular+outcomes+in+patients+with+coronary+artery+disease.">Echocardiographic measurements of epicardial adipose tissue and comparative ability to predict adverse cardiovascular outcomes in patients with coronary artery disease.</a> International Journal of Cardiovascular Imaging. 34 (9), 1429-1437 (2018).</li><li>Huang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelial+progenitor+cells+correlated+with+oxidative+stress+after+mild+traumatic+brain+injury.">Endothelial progenitor cells correlated with oxidative stress after mild traumatic brain injury.</a> Yonsei Medical Journal. 58 (5), 1012-1017 (2017).</li></ol>

从受影响的冠状动脉采集血液可能很困难。有时，冠状动脉几乎无法到达。在这种情况下，从静脉主塞采样可能是一种选择。我们进行了验证测试，比较冠状动脉中的循环生物标志物与静脉阴热，没有显著差异。然而，循环生物标志物的性能只用于冠状动脉取样。因此，从静脉主塞获得的生物标志物的性能仍有待探索。
最好在采血后的前 3 小时内处理 MpC 的样本。因此，心脏...

冠状血管化和支架是冠状动脉疾病 （CAD） 患者的抢救程序。然而，重大心血管不良事件（MACEs），包括心血管死亡、心肌梗死、冠状动脉恢复症和心绞痛发作或心力衰竭，可能在冠状动脉介入后数月发生，导致不定期到医院就诊。MACEs在全世界很普遍，其死亡率很高。
冠状缺血损伤诱导早期血管反应和重组机制，涉及动员MPC由于其分化能力和/或血管抑制电位，以及可溶性分子，如细胞间粘附分子（ICAM），基质金属蛋白酶（MMPs）和反应氧物种，反映细胞粘附，组织重塑和氧化应激。虽然内在血管特征，如斑块特征和冠状动脉复杂性已经被用来预测MACEs，一些研究表明，与冠状内皮发生损伤和修复机制相关的生物标志物对于CAD提交冠状血管成形的2、3、4、5患者的心血管事件的早期识别和预后非常有用。
持续的兴趣，了解CAD损伤和修复背后的机制，促使研究者研究冠状循环生物标志物，因为冠状动脉取样更密切地反映血管损伤和修复6。然而，在人类研究中，冠状生物标志物的表征一直很少，7、8、9。因此，本研究的目的是描述一种确定冠状循环MPC和可溶性分子的量的方法，反映血管损伤和修复，并表明这些生物标志物是否与MACE和接受冠状血管化的CAD患者的临床预后有关。该方法基于使用血管相关、循环的MpCs和可溶性分子，通过采样最接近血管损伤的位置获得。对于下肢缺血、中风、血管炎、静脉血栓和其他涉及血管损伤和修复的损伤的临床研究，它也可能有用。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>BSA</td>
			<td>Roche</td>
			<td>10735086001</td>
			<td>Bovine Serum Albumin (BSA) as a buffering agent, stabilizer, standard and for blending.</td>
		</tr>
		<tr>
			<td>Calibration Beads</td>
			<td>Miltenyi Biotec / MACS</td>
			<td>#130-093-607</td>
			<td>MACQuant calibration beads are supplied in aqueous solution containing 0.05% sodium azide. 3.5 ml for up to 100 tests</td>
		</tr>
		<tr>
			<td>CD133/1 (AC133)-PE</td>
			<td>Milteny Biotec / MACS</td>
			<td>#130-080-801</td>
			<td>Antibody conjugated to R-Phycoerythrin in PBS/EDTA buffer</td>
		</tr>
		<tr>
			<td>CD184 (CXCR4)-PE-VIO770</td>
			<td>Miltenyi Biotec / MACS</td>
			<td>#130-103-798</td>
			<td>Monoclonal, Isotype recombinant human IgG1, conjugated</td>
		</tr>
		<tr>
			<td>CD309 (VEGFR-2/KDR)-APC</td>
			<td>Miltenyi Biotec / MACS</td>
			<td>#130-093-601</td>
			<td>Antibody conjugated to R-Phycoerythrin in PBS/EDTA buffer</td>
		</tr>
		<tr>
			<td>CD34-FITC</td>
			<td>Miltenyi Biotec / MACS</td>
			<td>#130-081-001</td>
			<td>The monoclonal antibody clone AC136 detecs a class III epitope of the CD34</td>
		</tr>
		<tr>
			<td>CD45- VioBlue</td>
			<td>Miltenyi Biotec / MACS</td>
			<td>#130-092-880</td>
			<td>Monoclonal CD45 Antibody, human conjugated</td>
		</tr>
		<tr>
			<td>Conical Tubes</td>
			<td>Thermo SCIENTIFIC</td>
			<td>#339651</td>
			<td>15ml conical centrifuge tubes</td>
		</tr>
		<tr>
			<td>Cytometry Tubes</td>
			<td>FALCON Corning Brand</td>
			<td>#352052</td>
			<td>5 mL Polystyrene Round-Bottom Tube. 12x75 style. Sterile.</td>
		</tr>
		<tr>
			<td>EDTA</td>
			<td>BIO-RAD</td>
			<td>#161-0729</td>
			<td>Heavy metals, (as Pb) &#60;10ppm, Fe &#60;0.01%, As &#60;1ppm, Insolubles &#60;0.005%</td>
		</tr>
		<tr>
			<td>Improved Neubauer</td>
			<td>Without brand</td>
			<td>Without catalog number</td>
			<td>Hemocytometer for cell counting. (range 0.1000mm, 0.0025mm2)</td>
		</tr>
		<tr>
			<td>K2 EDTA Blood Collection Tubes</td>
			<td>BD Vacutainer</td>
			<td>#367863</td>
			<td>Lilac plastic vacutainer tube (K2E) 10.8mg, 6 mL.</td>
		</tr>
		<tr>
			<td>Lymphoprep</td>
			<td>Stemcell Technologies</td>
			<td>01-63-12-002-A</td>
			<td>Sterile and checked on the presence of endotoxins. Density: 1.077&#177;0.001g/mL</td>
		</tr>
		<tr>
			<td>Paraformaldehyde</td>
			<td>SIGMA-ALDRICH</td>
			<td>#SZBF0920V</td>
			<td>Fixation of biological samples, (powder, 95%)</td>
		</tr>
		<tr>
			<td>Pipette Transfer 1,3mL</td>
			<td>CRM Globe</td>
			<td>PF1016, PF1015</td>
			<td>The transfer pipette is a tool that facilitates liquid transfer with greater accuracy.</td>
		</tr>
		<tr>
			<td>Test Tubes</td>
			<td>KIMBLE CHASE</td>
			<td>45060 13100</td>
			<td>Heat-resistant test tubes. SIZE/CAP 13 x 100 mm</td>
		</tr>
	</tbody>
</table>

coronary progenitor cells and soluble biomarkers in cardiovascular prognosis after coronary angioplasty

重大心血管不良事件 （MACEs） 对冠状血管化患者的心血管预后产生负面影响，因为冠状缺血损伤。冠状动脉损伤的程度和血管修复机制是影响MACEs未来发展的因素。内血管特征，如斑块特征和冠状动脉复杂性已证明MACEs的预后信息。然而，冠状动脉内循环生物标志物的使用已被假定为早期识别和预后MACE的一种方便方法，因为它们更密切地反映了涉及冠状动脉损伤和修复的动态机制。血管成形术期间冠状循环生物标志物的测定，如单核祖细胞（MPCs）的亚种群数量，以及反映炎症、细胞粘附和修复的可溶性分子的浓度，允许评估未来的发展和MACEs的预后6个月冠状血管性血管性。该方法的转化性与外周血液循环生物标志物相比，在预测MACEs方面性能更好，对心血管预后的影响，可应用于患者的风险分层冠状动脉疾病经历血管性。

从52名接受冠状血管造影的患者中采集冠状、静脉坐宗和外周血（图1），显示高血压和血脂异常的高患病率。在临床随访中，11 （21.1%）在冠状血管造影术发生6个月后，MACEs：死亡（n = 1），需要住院治疗的心绞痛（n = 6），心肌梗死（n = 2），和/或心力衰竭的证据（n = 4）。
大多数MPC的基线冠状动脉浓度...

Watch this Scientific Journal Video about Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty at JoVE.com

Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty

严重不良心血管事件的发展，影响冠状血管后心血管预后，受冠状动脉损伤和血管修复程度的影响。使用新型冠状细胞和可溶性生物标志物，对血管损伤和修复反应，有助于预测MACEs的发展和预后。

冠状血管化后心血管预后冠状动脉祖细胞和可溶性生物标志物

Major adverse cardiovascular events (MACEs) negatively impact the cardiovascular prognosis of patients undergoing coronary angioplasty due to coronary ...

虽然血管成形术帮助冠状动脉疾病患者，但心血管事件仍可能发生在手术后。由于MPC和可溶性分子是从冠状动脉循环中获得的，它们可以与血管损伤和修复的程度相关。因此，冠状MPC和可溶性中介水平可用于估计冠状动脉疾病患者的心血管事件和预后，接受冠状动脉血管成形术。MPC 和可溶性分子也可用于估计在不同缺血环境中发生的并发症和预后，例如复发性肢体缺血性中风。演示该程序将是爱德华多·维拉-戈麦斯和亚历杭德罗·埃尔南德斯-帕特里西奥，实验室技术人员，卡伦·德拉维加-莫雷诺和卡洛斯·萨莫拉-阿莱曼，本科生。同样演示的还有科学硕士生加布里埃拉·亚历山德拉·多明格斯-佩雷斯、博士生阿尔贝托·梅尔乔-洛佩斯和科学硕士合作者马里奥·安东尼奥·泰列兹-冈萨雷斯。在采集后的一小时内，将六毫升采集的血液样本转移到15毫升锥形管中，并在PBS中以一比一的比例稀释血液。将两毫升密度梯度介质加入三个试管，并小心地将稀释的三个等等液转移到密度梯度介质的每个管中。通过离心分离细胞，并在接口将细胞转移到新管。将收集的细胞在两毫升PBS中清洗一次，每次洗涤6分钟，在两毫升新鲜PBS中清洗六次，每次洗涤两分钟。上次洗涤后，将含有MPC的细胞颗粒重新悬浮在PBS的一毫升中进行计数，并将1倍十添加到六个细胞中，以标记五毫升流动细胞学管。通过离心使细胞产生分裂，并加入100微升的合适抗体，稀释在抗体孵化溶液中，以每管。将细胞与每次添加的抗体鸡尾酒轻轻混合10秒，将样品放在4摄氏度，不受光线影响，20分钟。在孵化结束时，将样品离心，重新悬浮PBS 500微升颗粒，并辅以两毫摩尔EDTA。使用等型匹配对子来设置背景染色，根据标准方案通过流式细胞学分析样品，在一个正向的侧散射图中对淋巴细胞进行浇注，以排除残余粒细胞、细胞碎片和其他颗粒。设置一个门，以包含大量具有 CD45 阳性 CD34 阳性表型的细胞。接下来，使用CD45阳性CD34阳性门选择激酶插入域受体CD133或CD184阳性细胞。然后，MPC 子群体可以通过其特定的细胞表面标记进行标识，并报告为封闭事件的百分比。为了确定采集的血液样本中可溶性生物标志物的浓度，首先，用套件提供的洗涤缓冲液清洗适当的预涂 ELISA 板两次，并标记标准、样品和控制管。离心血液样本分离血液成分，将血浆分块分块分入样品管中。将标准、样品和控制装置转移到适当的孔中，并在 37 摄氏度下密封并孵育板 90 分钟。在孵化结束时，丢弃井内，将生物素检测抗体添加到每一个井中。在37摄氏度下孵育一小时后，丢弃盘子内装物，每次洗涤用新鲜洗涤缓冲液洗三次。上次洗涤后，用斯特雷普他丁工作溶液在37摄氏度下孵育样品30分钟，然后进行三次洗涤，如证明。最后一次洗涤后，使用四甲基苯胺基质在37摄氏度下处理样品30分钟。当颜色发展时，从套件中添加停止溶液，并在微孔 ELISA 读卡器中读取光密度吸收度。要确定肿瘤坏死因子α和白细胞间-1β的浓度，使用免疫磁性多路复用，标记标准、样品和控制管，并旋转磁珠小瓶30秒。将珠子添加到多路复用检测板的适当孔中。添加所有磁珠后，牢固插入手持式磁板垫圈，等待两分钟，让磁珠积聚在每一个井的底部，然后快速反转手持式磁板垫圈和废容器上的板组件。接下来，向每井添加 150 微升的洗涤缓冲液，等待 30 秒，让珠子积聚在底部。再次丢弃内容，正如刚刚演示的，并添加 25 微升的通用检测缓冲液和 25 微升的已准备标准、样品和控制。密封板至少60分钟孵育，在室温下不受光线影响，每分钟旋转500次。在孵育结束时，在每井中加入150微升的洗涤缓冲液，让珠子沉淀30秒。然后，使用板垫圈丢弃井内装物，并标注每井25微升检测抗体混合物，然后两次清洗，如证明。第二次洗涤后，用25微升的斯特雷普他丁PE孵育样品，在室温下孵育30分钟。在孵化结束时，在每口井中加入120微升的读数缓冲液，并密封板进行5分钟的孵育，在室温下不受光线影响，每分钟旋转500次。然后，将板加载到多路复用检测读取器上，并根据每个分析器调整读数参数。从52名接受冠状动脉血管造影术的患者中采集冠状动脉、静脉鼻窦和外周血，并证明高血压和血脂异常患病率很高。大多数MPC的基线冠状动脉浓度在出现重大心血管不良事件的患者中显著降低，MPC亚细胞CD34阳性CD133阳性和CD45阳性、CD34阳性、CD133阳性、CD184阳性的降幅较大。同样，出现重大心血管不良事件的患者在可溶性 ICAM-1 和较低量的金属蛋白 9 中增加了基线。冠状 MPC 和可溶性 ICAM-1 显示了主要心血管无不良生存的预后能力。有趣的是，肿瘤坏死因子α的表达根据测量时间和冠状动脉采样的位置，根据同一冠状动脉的不同流明区域的比较，使用血管内超声波显示变异。在MPC分离过程中，一定要将血液转移到密度梯度上，并在离心后小心地从界面收集细胞。周期性涡流可防止珠子沉淀，使用磁板垫圈有助于在洗涤过程中将珠子留在井内。冠状 MPC 和可溶性分子可用于在血管成形术后随发过程中降低心血管事件的风险，帮助向高危人群提供二级预防，因为此技术反映了停止溶液过程中发生的病理生理过程，对于血管生物学发挥作用的条件可能很有用。

Research

JoVE Journal

Medicine

Coronary Artery Ligation and Intramyocardial Injection in a Murine Model of Infarction

结扎冠状动脉内注射在小鼠模型梗死

Mouse models are a valuable tool for studying acute injury and chronic remodeling of the myocardium in vivo.  With the advent of genetic modifications to ...

科研

医学

Modified Technique for Coronary Artery Ligation in Mice

改性技术在小鼠冠状动脉结扎

Myocardial infarction (MI) is one of the most important causes of mortality in humans1-3. In order to improve morbidity and mortality in patients with MI ...

Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging

微型计算机断层扫描成像制备小鼠冠状动脉血管逆行灌注和灌装

Visualization of the vasculature is becoming increasingly important for understanding many different disease states. While several techniques exist for ...

Permanent Ligation of the Left Anterior Descending Coronary Artery in Mice: A Model of Post-myocardial Infarction Remodelling and Heart Failure

左冠状动脉前降支的小鼠结扎：对心肌梗死后重塑和心衰模型

Heart failure is a syndrome in which the heart fails to pump blood at a rate commensurate with cellular oxygen requirements at rest or during stress. It ...

Ultrasound Based Assessment of Coronary Artery Flow and Coronary Flow Reserve Using the Pressure Overload Model in Mice

冠脉流量和冠状动脉血流储备用小鼠压力超负荷基于模型的超声评估

Transthoracic Doppler echocardiography (TTDE) is a clinically useful, noninvasive tool for studying coronary artery flow velocity and coronary flow ...

Direct Re-implantation of Left Coronary Artery into the Aorta in Adults with Anomalous Origin of Left Coronary Artery from the Pulmonary Artery (ALCAPA)

Direct Re-implantation of Left Coronary Artery into the Aorta in Adults with Anomalous Origin of Left Coronary Artery from the Pulmonary Artery ALCAPA

左冠状动脉直接再注入与从肺动脉左冠状动脉起源异常成人主动脉（ALCAPA）

Anomalous origin of the left coronary artery from the pulmonary artery (ALCAPA) is a rare congenital anomaly which is one of leading causes of myocardial ...

Identifying Coronary Artery Calcification on Non-gated Computed Tomography Scans

非门控断层扫描的冠状动脉钙化识别

Coronary artery calcification (CAC) provides an objective measure of coronary artery disease and can readily be identified on non-gated computed ...

Evaluation of Coronary Flow Reserve After Myocardial Ischemia Reperfusion in Rats

大鼠心肌缺血再灌注后冠状动脉流动储备评价

Coronary artery disease is the leading cause of death worldwide. After an acute myocardial infarction, early and successful myocardial intervention via ...

In Vitro Model of Coronary Angiogenesis

冠状血管生成体模型

Here, we describe an in vitro culture assay to study coronary angiogenesis. Coronary vessels feed the heart muscle and are of clinical importance. Defects ...

Novel Percutaneous Approach for Deployment of 3D Printed Coronary Stenosis Implants in Swine Models of Ischemic Heart Disease

在缺血性心脏病的猪模型中部署3D打印冠状动脉狭窄植入物的新颖的皮下方法

Minimally invasive methods for creating models of focal coronary narrowing in large animals are challenging. Rapid prototyping using three-dimensionally ...