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DOI :
10.3791/60507-v
•
10:12 min
December 20th, 2019
Chapters
0:04
Title
0:46
Collagen Sandwich Preparation
1:40
Liver Harvest
4:04
Primary Hepatocyte Isolation
5:07
3D Collagen Sandwich Primary Hepatocyte Culture
6:11
Immunolabeling
7:47
Results: Representative Assessment of Cytoskeleton Function in In Vitro Bile Canalicular Formation
9:34
Conclusion
ここで提示するプロトコルは、修飾コラゲ素灌流技術を用いた成体マウス肝臓からのマウス肝細胞の単離のためのプロトコルである。また、3Dコラーゲンサンドイッチ設定における肝細胞の長期培養と、胆道形成および治療に対する応答を研究するための細胞骨格成分の免疫標識についても説明する。
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