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DOI :
10.3791/60533-v
•
10:27 min
December 5th, 2019
Chapters
0:04
Title
1:04
Site-directed Mutagenesis
2:29
Recombinant Protein Purification and In Vitro Ubiquitination Assay
3:40
Agrobacterium-mediated Transient Protein Expression in Nicotiana benthamiana Leaves and In Planta Ubiquitination Assay
6:36
Establishing the Link Between Enzymatic Activity and Function In Planta
7:27
Results: In Vitro and In Planta Ubiquitination Assay
9:20
Conclusion
이 원고의 목표는 RING 형 E3 유비퀴틴 리가제의 포괄적 인 생화학 및 기능 적 연구에 대한 개요를 제시하는 것입니다. 상세한 프로토콜을 갖춘 이 다단계 파이프라인은 테스트된 단백질의 효소 활성을 검증하고 활성을 작동하도록 연결하는 방법을 보여줍니다.
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