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DOI :
10.3791/60588-v
•
6:49 min
January 14th, 2020
Chapters
0:04
Title
0:44
Brain Tissue Recovery and Meninges Removal
1:31
Homogenization
2:18
Vascular Fraction Isolation
4:32
Cerebral Pericyte Proliferation
5:25
Results: Representative Cell Morphology, Marker Expression, and Culture Purity
6:25
Conclusion
我々は、マウス脳ペリサイトの抽出のためのプロトコルを提示する。抗生物質フリー濃縮指向のペリサイト抽出に基づいて、このプロトコルは、高純度と高収率を提供するインビトロ研究のための貴重なツールであり、したがって、使用される実験動物の数を減少させる。
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