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DOI :
10.3791/60660-v
•
7:20 min
February 28th, 2020
Chapters
0:04
Introduction
0:51
Melanoma Cell and Skin Fibroblast Culture
1:36
Melanoma Cell and Fibroblast Fluorescent Lentivirus Transfection
2:22
Melanoma Cell:Fibroblast Co-Culture and Melanoma Cell Solo Culture
3:25
Live Cell Time-Lapse Imaging
4:22
Confocal Microscopic 3D Movie Generation
4:59
Results: Representative 3D Tumor Spheroid Formation and Analysis
6:32
Conclusion
建立了基于肿瘤细胞和基质成纤维细胞异质相互作用的新型三维球体模型。在这里,我们介绍肿瘤细胞和基质成纤维细胞的共培养,延时成像和共聚焦显微镜,以可视化球形的形成。该三维模型为研究肿瘤-频闪相互作用和测试癌症治疗提供了一个相关的平台。
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