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DOI :
10.3791/60709-v
•
10:40 min
June 5th, 2020
Chapters
0:04
Introduction
0:46
Organoid Recovery
2:28
Fixation and Blocking
3:30
Immunolabeling
5:33
Optical Clearing
6:08
Confocal Imaging Slide Preparation
7:01
Image Acquisition and Processing
8:06
Results: Representative 3D Imaging and Organoid Clearing
10:01
Conclusion
オルガノイドの全体の3D構造および細胞内容、ならびに元の組織に似たその表現型は、ここで説明する単一細胞分解能3D画像化プロトコルを使用して捕捉することができる。このプロトコルは、起源、大きさおよび形状が変化するオルガノイドの広い範囲に適用することができる。
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