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DOI :
10.3791/60712-v
January 25th, 2020
Chapters
0:04
Title
0:45
DNA Probe Preparation and Nick Translation Labelling
2:15
Human Primary T Cell Fixation, Pre-Treatment, and Permeabilization
4:50
Human Primary Myoblast Cell Fixation, Pre-Treatment, and Permeabilization
5:43
3D multicolor DNA FISH
7:05
3D Multicolor DNA FISH Detection and Analysis
8:52
Results: Representative DNA FISH Analysis of Human Primary Cell Nuclear Architecture
10:45
Conclusion
3D 다색 DNA FISH는 3D 보존 핵 내에서 다중 게놈 궤시를 시각화하는 도구를 나타내며, 단일 세포 수준에서 핵 공간 내에서 상호 상호 작용 및 국소화를 명확하게 정의합니다. 여기서, 단계별 프로토콜은 인간 1차 세포의 광범위한 스펙트럼에 대해 기재된다.
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