Name: lower limb biomechanical analysis of healthy participants
Uploaded: 2020-04-15T14:00:00
Description: Watch this Scientific Journal Video about Lower Limb Biomechanical Analysis of Healthy Participants at JoVE.com

我们要感谢约翰纳森·威廉姆斯博士对MATLAB数据处理的建议。

在一项获得伯恩茅斯大学研究伦理委员会（参考15005）的道德批准下，对所报告的方法进行了跟踪研究。
1. 参与者
<ol><li>招募健康成年人（年龄从23岁到63岁，均值=S.D.;42.0= 13.4，体重70.4×15.3公斤，身高175.5±9.8厘米;15名男性，15名女性）参加研究。为这项研究招募了30名参与者。</li>
	<li>确保参与者没有自报头晕、平衡问题或行走困难史。</li>
	<li>确保参与者不会遭受任何已知?...

<ol>
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H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+method+for+the+reduction+of+skin+marker+artifacts+during+walking+:+Application+to+the+knee.">A method for the reduction of skin marker artifacts during walking : Application to the knee.</a> KSME International Journal. 17 (6), 825-835 (2003).</li><li>Liu, P. C., Liu, J. F., Chen, L. Y., Xia, K., Wu, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intermittent+pneumatic+compression+devices+combined+with+anticoagulants+for+prevention+of+symptomatic+deep+vein+thrombosis+after+total+knee+arthroplasty:+a+pilot+study.">Intermittent pneumatic compression devices combined with anticoagulants for prevention of symptomatic deep vein thrombosis after total knee arthroplasty: a pilot study.</a> Therapeutics and Clinical Risk Management. 13, 179-183 (2017).</li><li>Al-Amri, M., Al Balushi, H., Mashabi, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intra-rater+repeatability+of+gait+parameters+in+healthy+adults+during+self-paced+treadmill-based+virtual+reality+walking.">Intra-rater repeatability of gait parameters in healthy adults during self-paced treadmill-based virtual reality walking.</a> Computer Methods in Biomechanics and Biomedical Engineering. 20 (16), 1669-1677 (2017).</li><li>Zeni, J., Richards, J., Higginson, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two+simple+methods+for+determining+gait+events+during+treadmill+and+overground+walking+using+kinematic+data.">Two simple methods for determining gait events during treadmill and overground walking using kinematic data.</a> Gait &amp; Posture. 27 (4), 710-714 (2008).</li><li>Tsaopoulos, D. E., Baltzopoulos, V., Richards, P. J., Maganaris, C. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanical+correction+of+dynamometer+moment+for+the+effects+of+segment+motion+during+isometric+knee-extension+tests.">Mechanical correction of dynamometer moment for the effects of segment motion during isometric knee-extension tests.</a> Journal of Applied Physiology. 111 (1), 68-74 (2011).</li><li>Abernethy, P., Wilson, G., Logan, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Strength+and+power+assessment.+Issues,+controversies+and+challenges.">Strength and power assessment. Issues, controversies and challenges.</a> Sports Medicine. 19 (6), 401-417 (1995).</li><li>Kroll, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reliability+of+a+Selected+Measure+of+Human+Strength.">Reliability of a Selected Measure of Human Strength.</a> Research Quarterly, American Association for Health, Physical Education and Recreation. 33 (3), 410-417 (1962).</li><li>Anzak, A., Tan, H., Pogosyan, A., Brown, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Doing+better+than+your+best:+loud+auditory+stimulation+yields+improvements+in+maximal+voluntary+force.">Doing better than your best: loud auditory stimulation yields improvements in maximal voluntary force.</a> Experimental Brain Research. 208 (2), 237-243 (2011).</li><li>Belkhiria, C., De Marco, G., Driss, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+verbal+encouragement+on+force+and+electromyographic+activations+during+exercise.">Effects of verbal encouragement on force and electromyographic activations during exercise.</a> Journal of Sports Medicine and Physical Fitness. 58 (5), 750-757 (2018).</li><li>Bickers, M. 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这项研究的贡献是在一个协议中准确和全面地描述以前没有一起描述的步态分析和肌肉力量测试相结合的技术。
为了获得步态分析的准确结果，有两个方面需要最大的关注：1） 标记放置和 2） 适应时间。测量数据的准确性在很大程度上取决于所用模型的准确性。影响准确性的其他关键因素包括相对于底层骨骼结构的表面性皮肤变形引起的错误标记运动，以及跟踪系统的分辨...

生物力学的学科主要涉及研究压力、应变、载荷和生物系统的运动——固体和流体。它还涉及对车身结构、尺寸、形状和运动的机械效应的建模。多年来，这一领域的发展提高了我们对正常和病理步态、神经肌肉控制机制、生长机制和2型机学的理解。
本文的主要目标是介绍一种综合方法，介绍两种可用于测量个体下肢生物力学的最新技术。步态分析系统测量和量化步态生物力学通过使用自定速 （SP） 跑步机与增强现实环境相结合，该增强现实环境集成了 SP 算法来调节跑步机的速度，如 Sloot 等人3所述。肌肉力量测试设备被用作上肢康复的评估和治疗工具4。该装置可以客观地评估等轴测和等距模式下的各种运动生理模式或工作模拟任务。它目前被认为是上肢力量测量的黄金标准5，但与下肢具体有关的证据仍然不清楚。本文阐述了完成下肢步态和等轴测强度评估的详细方案。
在生物力学分析中，将功能性能评估（如步态分析）与肌肉性能的特定测试相结合是很有用的。这是因为，虽然可以假设增加肌肉力量提高功能性能，这可能并不总是明显的6。这种理解是改进康复方案及研究战略的未来设计，以评估这些方法所必需的。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>701 Small lever</td>
			<td>Baltimore Therapeutic Equipment Company (BTE)</td>
			<td>Not Available - Online link provided in description</td>
			<td>The unique attachment designed for the Primus RS to measure Knee Extension/Flexion - <a href="https://store.btetech.com/collections/primus/products/701-small-lever" target="_blank">https://store.btetech.com/collections/primus/products/701-small-lever</a></td>
		</tr>
		<tr>
			<td>D-Flow Software - Vresion 3.26</td>
			<td>Motekforce Link</td>
			<td>Not Available - Online link provided in description</td>
			<td>Software used to control GRAIL system - <a href="https://summitmedsci.co.uk/products/motek-dflow-hbm-software/" target="_blank">https://summitmedsci.co.uk/products/motek-dflow-hbm-software/</a></td>
		</tr>
		<tr>
			<td>Gait Offline Analysis (GOAT) - Version 2.3</td>
			<td>Motekforce Link</td>
			<td>Not Available - Online link provided in description</td>
			<td>Software used for the analysis of the gait parameters - <a href="https://www.motekmedical.com/product/grail/" target="_blank">https://www.motekmedical.com/product/grail/</a></td>
		</tr>
		<tr>
			<td>Gait Real-time Analysis Interactive Lab (GRAIL)</td>
			<td>Motekforce Link</td>
			<td>Not Available - Online link provided in description</td>
			<td>GRAIL system measures and quantifies gait biomechanics by using a virtual reality based self-paced (SP) treadmill - <a href="https://www.motekmedical.com/product/grail/" target="_blank">https://www.motekmedical.com/product/grail/</a></td>
		</tr>
		<tr>
			<td>Leg Pad for 701</td>
			<td>Baltimore Therapeutic Equipment Company (BTE)</td>
			<td>Not Available - Online link provided in description</td>
			<td>The unique attachment designed for the Primus RS to measure Knee Extension/Flexion - <a href="https://store.btetech.com/collections/primus/products/701-802-leg-pad" target="_blank">https://store.btetech.com/collections/primus/products/701-802-leg-pad</a></td>
		</tr>
		<tr>
			<td>Positioning Chair</td>
			<td>Baltimore Therapeutic Equipment Company (BTE)</td>
			<td>Not Available - Online link provided in description</td>
			<td>Participant Positioning Chair is designed for assessment and treatment of the lower exteremeties. The chair is designed for multiple positions. <a href="https://www.btetech.com/product/primus/" target="_blank">https://www.btetech.com/product/primus/</a></td>
		</tr>
		<tr>
			<td>Primus RS</td>
			<td>Baltimore Therapeutic Equipment Company (BTE)</td>
			<td>Not Available - Online link provided in description</td>
			<td>Primus RS equipment captures and reports real time objective data in Isotonic, Isometric, and Isokinetic resistance modes - <a href="https://www.btetech.com/wp-content/uploads/BTE-Rehabilitation-Equipment-PrimusRS-Brochure-1.pdf" target="_blank">https://www.btetech.com/wp-content/uploads/BTE-Rehabilitation-Equipment-PrimusRS-Brochure-1.pdf</a></td>
		</tr>
	</tbody>
</table>

lower limb biomechanical analysis of healthy participants

生物力学分析技术在研究人类运动方面非常有用。本研究的目的是在使用商业可用的系统向健康参与者引入下肢生物力学评估技术。为步态分析和肌肉力量测试系统引入了单独的协议。为确保步态评估的最大准确性，应注意标记位置和自定进度的跑步机适应时间。同样，参与者定位、练习试验和言语鼓励是肌肉力量测试的三个关键阶段。目前的证据表明，本文概述的方法可能对下肢生物力学的评估有效。

表2给出了时空、运动学和动态步态参数的均值和标准差。MVIC所有30名参与者的数据都汇总在表3中。图 4和图 5分别提供了一个参与者左侧和右侧的典型数据集，其中显示了步态参数的图形表示形式。
所提交的数据代表了所有参与者获得的结果，并且与为步?...

Watch this Scientific Journal Video about Lower Limb Biomechanical Analysis of Healthy Participants at JoVE.com

Lower Limb Biomechanical Analysis of Healthy Participants

本文介绍了一种全面的实验方法，对两种可用于测量个体下肢生物力学的最新技术进行了测量。

健康参与者的下肢生物力学分析

Biomechanical analysis techniques are useful in the study of human movement. The aim of this study was to introduce a technique for the lower limb ...

生物力学分析技术在研究人类运动方面是有用的。该技术可用于使用市售系统对健康参与者进行生物力学评估。在生物力学分析中，对步态分析等功能进行特定测试，结合肌肉性能测试是很有用的。在未来的研究研究和康复方案的设计中，需要了解肌肉力量与执行诸如行走等功能任务的能力之间的关系。今天展示我们协议的是弗朗切斯科·费拉罗，我们团队的试用经理。在开始分析之前，确认参与者穿着紧身非反光服装，如自行车短裤或打底裤，并使用双面胶带根据人体模型的下半身结构将 25 个被动反射标记连接到参与者身上。如图所示。使用关节标尺测量模型所需的膝盖和脚踝宽度，并保护学员固定在固定到头顶框架上的安全带。在数据库中启动新会话，并确保该会话处于活动状态。在"主题"选项卡下，单击"标记骨架"按钮并导航到下肢 HBM N2 VST 文件以创建新参与者。输入参与者的名称，然后单击"确定"。参与者的姓名将显示在主题窗口中。在工具窗口中，打开"主题准备"选项卡。单击将力板归零，并要求学员站在跑步机中间，同时在适应后以舒适的速度行走五分钟，然后单击软件中的"开始录制"开始录制步态数据。获取所需数据量后停止录制。要消除数据中的高频噪声，请打开处理软件，为标记数据选择一个低通滤波器，并使用脚标记确定增益事件，从垂直力数据中确定单个步幅。将数据导出为 CSV 文件。然后可以分析步态参数，如运动学、动力学和空间时态数据。要根据最大自愿等轴收缩测量参与者的肌肉力量，请先将工具垫编号 701 连接到测功机练习头，让参与者坐在椅子上休息。要测试膝盖等轴测量肌肉强度，请使用上下开关将测功机轴与膝关节旋转的解剖轴对齐，并将工具垫中央放在头骨小腿的下部。让参与者将膝盖定位为 90 度弯曲，臀部处于中性旋转和诱拐，脚在植物弯曲中。然后让参与者将手放在腹部，用尼龙搭扣带将躯干、臀部和大腿中部稳定在椅子上。要让学员熟悉测试操作，让学员在弯曲前伸展膝盖，在命令上施加最大收缩三秒钟，同时提供口头提示和鼓励。当学员对手术感到自在时，确保学员在遇到任何异常疼痛或不适时可以立即停止测试，并允许学员休息两分钟。然后重复试验三次左腿和三次右腿，只是演示记录和导出牛顿的数据。在这里，显示了30名参与者在代表性分析中的空间时间、运动学和动能步态参数的均值和标准偏差。如本表所见，总结了参与者膝关节最大自愿等轴收缩的均值和标准偏差，平均而言，右膝在评估参与者中表现出稍微强一些的延伸和弯曲。在一个参与者的步态评估的离线分析中，可以观察到参与者左侧的空间时间数据以及运动和动能步态周期。在评估参与者右侧的空间时态数据以及运动和动能步态循环时，这些数据同样被转载。临床研究已经确定了人类生物力学特征与不同医疗条件之间的明确联系。该协议可用于准确和全面测试组合步态分析和肌肉力量的方式，这是以前没有描述过的。未来的骨科研究将需要准确和可重复的技术来测量步态。通过所述技术收集的数据与用于步态分析和等轴测肌肉力量测试的国际参考数据一致。

Research

JoVE Journal

Medicine

Tilt Testing with Combined Lower Body Negative Pressure: a "Gold Standard" for Measuring Orthostatic Tolerance

倾斜试验结合下体负压测量立位耐力的“金标准”

Orthostatic tolerance (OT)  refers to the ability to maintain cardiovascular stability when upright,  against the hydrostatic effects of gravity, and ...

科研

医学

Bladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology

膀胱平滑肌收缩带作为一种方法来评估下尿路药理学

We describe an in vitro method to measure bladder smooth muscle contractility, and its use for investigating physiological and pharmacological properties ...

Remote Limb Ischemic Preconditioning:  A Neuroprotective Technique in Rodents

远程肢体缺血预适应：在啮齿动物的保护技术

Sublethal ischemia protects tissues against subsequent, more severe ischemia through the upregulation of endogenous mechanisms in the affected tissue. ...

Orthotopic Hind Limb Transplantation in the Mouse

原位后肢移植中的鼠标

In vivo animal model systems, and in particular mouse models, have evolved into powerful and versatile scientific tools indispensable to basic and ...

TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents

TRUE基因沉默：一个七聚物型小导RNA库的筛选潜在的癌症治疗药

TRUE gene silencing (termed after tRNase ZL-utilizing efficacious gene silencing) is one of the RNA-directed gene silencing technologies, which utilizes ...

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

口腔生物膜取样在健康儿童中的微生物分析

Oral biofilm and its molecular analysis provide a basis for investigating various dental research and clinical questions. Knowledge of biofilm composition ...

Assessment of the Efficacy of An Osteopathic Treatment in Infants with Biomechanical Impairments to Suckling

Breastfeeding can be challenging for mother-infant dyads experiencing biomechanical suckling difficulties. Although lactation consultants (LCs) all over ...

Preclinical Model of Hind Limb Ischemia in Diabetic Rabbits

糖尿病家兔欣德肢体缺血的临床前模型

Peripheral vascular disease is a widespread clinical problem that affects millions of patients worldwide. A major consequence of peripheral vascular ...

The Lower Body Positive Pressure Treadmill for Knee Osteoarthritis Rehabilitation

下体正压跑步机用于膝关节骨关节炎康复

Here, based on a clinician&rsquo;s point-of-view, we propose a two-model lower body positive pressure (LBPP) protocol (walking and squatting models) in ...

Virtual Prism Adaptation Therapy: Protocol for Validation in Healthy Adults

虚拟棱镜适应疗法：健康成人验证协议

Hemispatial neglect is a common impairment after stroke. It is associated with poor functional and social outcomes. Therefore, an adequate intervention is ...