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DOI :
10.3791/60750-v
•
9:33 min
April 2nd, 2020
Chapters
0:04
Introduction
1:04
Prefilling of Chemotaxis Slides
2:25
Isolation of Mouse Resident Peritoneal Macrophages
3:40
Seeding Peritoneal Cells and Adding Chemoattractant
7:31
Results: Chemotaxis of Mouse Macrophages
9:07
Conclusion
在这里,我们描述了使用延时,相位对比显微镜成像小鼠常驻腹腔巨噬细胞在化学策略补充C5a梯度的方法。协议可以扩展到其他免疫细胞。
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