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DOI :
10.3791/60840-v
•
6:20 min
May 7th, 2020
Chapters
0:04
Introduction
0:54
Isolation and Primary Culture of Urine-Derived Cells
2:31
Infection with MYOD1-Retroviral Vector
3:20
Myogenic Differentiation of MYOD1-Transduced UDCs
3:53
Evaluation of Exon Skipping Efficiency by RT-PCR
5:05
Results: Restoration of Dystrophin mRNA in UDCs
5:57
Conclusion
在本文中,我们描述了一个详细的协议,使用MYOD1- 转化的尿液衍生细胞有效建模Duchenne肌肉营养不良肌,以评估脱外器跳过后肌营养不良的mRNA和蛋白质水平的恢复。
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