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DOI :
10.3791/60840-v
•
6:20 min
May 7th, 2020
Chapters
0:04
Introduction
0:54
Isolation and Primary Culture of Urine-Derived Cells
2:31
Infection with MYOD1-Retroviral Vector
3:20
Myogenic Differentiation of MYOD1-Transduced UDCs
3:53
Evaluation of Exon Skipping Efficiency by RT-PCR
5:05
Results: Restoration of Dystrophin mRNA in UDCs
5:57
Conclusion
이 문서에서는, 우리는 EXON 건너뛰기 후에 dystrophin mRNA 및 단백질 수준의 회복을 평가하기 위하여 MYOD1-변환된소변 유래 세포를 사용하여 Duchenne 근이영양증 근육의 능률적인 모델링을 위한 상세한 프로토콜을 기술합니다.
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