Name: stimulation of stem cell niches and tissue regeneration in mouse skin by switchable protoporphyrin ix-dependent photogeneration of reactive oxygen species in situ
Uploaded: 2020-05-08T12:30:00
Description: Watch this Scientific Journal Video about Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In

Dette arbejde er blevet støttet af tilskud fra Ministerio de Economía y Competitividad (RTC-2014-2626-1 til JE) og Instituto de Salud Carlos III (PI15/01458 til JE) i Spanien. EF er blevet støttet af Atracción de Talento Investigador-tilskuddet 2017-T2/BMD-5766 (Comunidad de Madrid og UAM).

Alle musehold og forsøg skal udføres i overensstemmelse med lokal, national, international lovgivning og retningslinjer for dyreforsøg.
1. Induktion af hårvækst, forbrænding, induktion og identifikation af langsigtede BrdU LRC'er i hele halehudens epitel
BEMÆRK: Brug 10 dage eller 7 uger gamle C57BL/6-mus, helst kuldkammerater, til de eksperimentelle designs, der er beskrevet nedenfor. I alle forsøgsprocedurer vil dyrene blive bedøvet ved 3% isofluranindåndi...

<ol>
	<li>Bl&#225;zquez-Castro, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direct+1O2+optical+excitation:+A+tool+for+redox+biology.">Direct 1O2 optical excitation: A tool for redox biology.</a> Redox Biology. 13, 39-59 (2017).</li><li>Valko, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Free+radicals+and+antioxidants+in+normal+physiological+functions+and+human+disease.">Free radicals and antioxidants in normal physiological functions and human disease.</a> The International Journal of Biochemistry &amp; Cell Biology. 39 (1), 44-84 (2007).</li><li>Sena, L. A., Chandel, N. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Physiological+Roles+of+Mitochondrial+Reactive+Oxygen+Species.">Physiological Roles of Mitochondrial Reactive Oxygen Species.</a> Molecular Cell. 48 (2), 158-167 (2012).</li><li>Bartosz, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reactive+oxygen+species:+Destroyers+or+messengers.">Reactive oxygen species: Destroyers or messengers.</a> Biochemical Pharmacology. 77 (8), 1303-1315 (2009).</li><li>Brieger, K., Schiavone, S., Miller, J., Krause, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reactive+oxygen+species:+from+health+to+disease.">Reactive oxygen species: from health to disease.</a> Swiss Medical Weekly. 142, 13659 (2012).</li><li>Speakman, J. R., Selman, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+free-radical+damage+theory:+Accumulating+evidence+against+a+simple+link+of+oxidative+stress+to+ageing+and+lifespan.">The free-radical damage theory: Accumulating evidence against a simple link of oxidative stress to ageing and lifespan.</a> BioEssays. 33 (4), 255-259 (2011).</li><li>Fernandez, V., Videla, L. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biochemical+aspects+of+cellular+antioxidant+systems.">Biochemical aspects of cellular antioxidant systems.</a> Biological Research. 29 (2), 177-182 (1996).</li><li>Mat&#233;s, J. M., S&#225;nchez-Jim&#233;nez, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antioxidant+enzymes+and+their+implications+in+pathophysiologic+processes.">Antioxidant enzymes and their implications in pathophysiologic processes.</a> Frontiers in Bioscience. 4, 339-345 (1999).</li><li>Bedard, K., Krause, K. -. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+NOX+Family+of+ROS-Generating+NADPH+Oxidases:+Physiology+and+Pathophysiology.">The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology.</a> Physiological Reviews. 87 (1), 245-313 (2007).</li><li>Leto, T. L., Morand, S., Hurt, D., Ueyama, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeting+and+Regulation+of+Reactive+Oxygen+Species+Generation+by+Nox+Family+NADPH+Oxidases.">Targeting and Regulation of Reactive Oxygen Species Generation by Nox Family NADPH Oxidases.</a> Antioxidants &amp; Redox Signaling. 11 (10), 2607-2619 (2009).</li><li>Hern&#225;ndez-Garc&#237;a, D., Wood, C. D., Castro-Obreg&#243;n, S., Covarrubias, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reactive+oxygen+species:+A+radical+role+in+development.">Reactive oxygen species: A radical role in development.</a> Free Radical Biology and Medicine. 49 (2), 130-143 (2010).</li><li>Covarrubias, L., Hern&#225;ndez-Garc&#237;a, D., Schnabel, D., Salas-Vidal, E., Castro-Obreg&#243;n, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Function+of+reactive+oxygen+species+during+animal+development:+Passive+or+active.">Function of reactive oxygen species during animal development: Passive or active.</a> Developmental Biology. 320 (1), 1-11 (2008).</li><li>Timme-Laragy, A. R., Hahn, M. E., Hansen, J. M., Rastogi, A., Roy, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Redox+stress+and+signaling+during+vertebrate+embryonic+development:+Regulation+and+responses.">Redox stress and signaling during vertebrate embryonic development: Regulation and responses.</a> Seminars in Cell &amp; Developmental Biology. 80, 17-28 (2018).</li><li>Owusu-Ansah, E., Banerjee, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reactive+oxygen+species+prime+Drosophila+haematopoietic+progenitors+for+differentiation.">Reactive oxygen species prime Drosophila haematopoietic progenitors for differentiation.</a> Nature. 461 (7263), 537-541 (2009).</li><li>Love, N. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Amputation-induced+reactive+oxygen+species+are+required+for+successful+Xenopus+tadpole+tail+regeneration.">Amputation-induced reactive oxygen species are required for successful Xenopus tadpole tail regeneration.</a> Nature Cell Biology. 15 (2), 222-228 (2013).</li><li>Le Belle, J. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proliferative+Neural+Stem+Cells+Have+High+Endogenous+ROS+Levels+that+Regulate+Self-Renewal+and+Neurogenesis+in+a+PI3K/Akt-Dependant+Manner.">Proliferative Neural Stem Cells Have High Endogenous ROS Levels that Regulate Self-Renewal and Neurogenesis in a PI3K/Akt-Dependant Manner.</a> Cell Stem Cell. 8 (1), 59-71 (2011).</li><li>Myant, K. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=production+and+NF-&#954;B+activation+triggered+by+RAC1+facilitate+WNT-driven+intestinal+stem+cell+proliferation+and+colorectal+cancer+initiation.">production and NF-&#954;B activation triggered by RAC1 facilitate WNT-driven intestinal stem cell proliferation and colorectal cancer initiation.</a> Cell Stem Cell. 12 (6), 761-773 (2013).</li><li>Hamanaka, R. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mitochondrial+Reactive+Oxygen+Species+Promote+Epidermal+Differentiation+and+Hair+Follicle+Development.">Mitochondrial Reactive Oxygen Species Promote Epidermal Differentiation and Hair Follicle Development.</a> Science Signaling. 6 (261), 8 (2013).</li><li>Carrasco, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Photoactivation+of+ROS+Production+in+situ+Transiently+Activates+Cell+Proliferation+in+Mouse+Skin+and+in+the+hair+Follicle+Stem+Cell+Niche+Promoting+Hair+Growth+and+Wound+Healing.">Photoactivation of ROS Production in situ Transiently Activates Cell Proliferation in Mouse Skin and in the hair Follicle Stem Cell Niche Promoting Hair Growth and Wound Healing.</a> Journal of Investigative Dermatology. 135 (11), 1-12 (2015).</li><li>Carrasco, E., Bl&#225;zquez-Castro, A., Calvo, M. I., Juarranz, &. #. 1. 9. 3. ;., Espada, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Switching+on+a+transient+endogenous+ROS+production+in+mammalian+cells+and+tissues.">Switching on a transient endogenous ROS production in mammalian cells and tissues.</a> Methods. , 109 (2016).</li><li>Braun, K. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Manipulation+of+stem+cell+proliferation+and+lineage+commitment:+visualisation+of+label-retaining+cells+in+wholemounts+of+mouse+epidermis.">Manipulation of stem cell proliferation and lineage commitment: visualisation of label-retaining cells in wholemounts of mouse epidermis.</a> Development. 130 (21), 5241-5255 (2003).</li><li>Hsu, Y. -. C., Li, L., Fuchs, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Emerging+interactions+between+skin+stem+cells+and+their+niches.">Emerging interactions between skin stem cells and their niches.</a> Nature Medicine. 20 (8), 847-856 (2014).</li><li>Plikus, M. V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epithelial+stem+cells+and+implications+for+wound+repair.">Epithelial stem cells and implications for wound repair.</a> Seminars in Cell &amp; Developmental Biology. 23 (9), 946-953 (2012).</li></ol>

Her præsenterer vi en metode, der tillader en forbigående aktivering af endogen ROS-produktion in vivo i musehud med fysiologiske virkninger. Metoden er baseret på en fotodynamisk procedure til at inducere en kontrolleret og lokal stimulering af det endogene fotosensibilisator PpIX (figur 1B). Denne eksperimentelle tilgang er et interessant værktøj til at studere ROS-biologi i in vivo-eksperimentelle systemer, der udgør et betydeligt fremskridt i forhold til metoder, der bruger ekstern...

Reaktive iltarter (ROS) er resultatet af den kemiske reduktion af molekylært ilt til dannelse af vand og inkluderer singlet oxygen, superoxidanion, hydrogenperoxid og hydroxylradikalet 1,2,3. ROS har en meget kort levetid på grund af deres ekstremt kemiske reaktive natur. I aerobe organismer dannes ROS tilfældigvis inde i cellerne som et stort utæt biprodukt af aerob respiration (elektrontransportkæde) i mitokondrierne. Forbigående akkumulering af høje niveauer af ROS i cellen resulterer i en oxidativ stresstilstand, der kan fremkalde irreversibel inaktivering af proteiner, lipider og sukkerarter og introduktion af mutationer i DNA-molekylet 2,3,4,5. Den gradvise akkumulering af oxidativ skade i celler, væv og hele organismer stiger støt med tiden og har været forbundet med induktion af celledødsprogrammer, flere patologier og aldringsprocessen 2,3,4,6.
Aerobe organismer har støt udviklet effektive molekylære mekanismer til at tackle overskydende ROS-akkumulering i celler og væv. Disse mekanismer omfatter medlemmer af superoxiddismutase (SOD) proteinfamilien, som katalyserer superoxidradikal dismutation til molekylært ilt og hydrogenperoxid, samt forskellige katalaser og peroxidaser, der bruger antioxidantpuljen (glutathion, NADPH, peroxiredoxin, thioredoxin 7,8) til at katalysere den efterfølgende omdannelse af hydrogenperoxid til vand og molekylært oxygen.
Imidlertid understøtter flere rapporter ROS's rolle som nøglekomponenter i molekylære kredsløb, der regulerer kritiske cellefunktioner, herunder spredning, differentiering og mobilitet 2,3,4. Dette koncept understøttes yderligere af den indledende identifikation og karakterisering af dedikerede ROS-producerende mekanismer i aerobe organismer, herunder lipoxygenaser cyclooxygenaser og NADPH-oxidaser 9,10. I denne forstand udviser ROS en aktiv rolle under hvirveldyrs embryoudvikling 11,12,13, og nøgleroller for disse molekyler i reguleringen af specifikke in vivo fysiologiske funktioner er blevet rapporteret i forskellige eksperimentelle systemer, herunder differentieringsprogrammet for hæmatopoietiske forfædre i Drosophila14, helbredende induktion i zebrafisk eller haleregenerering i Xenopus haletudser 15. Hos pattedyr har ROS været involveret i neurale stamcellers selvfornyelse/differentieringspotentiale i en neurosfæremodel16 og i dereguleringen af tarmens stamcellefunktion under initiering af kolorektal cancer17. I huden har ROS-signalering været forbundet med epidermal differentiering og regulering af hudens stamcelleniche og hårsækkens vækstcyklus18,19.
I dette perspektiv er en væsentlig eksperimentel begrænsning for at bestemme ROS's fysiologiske roller i biologiske systemer, både under normale eller patologiske forhold, manglen på tilstrækkelige eksperimentelle værktøjer til at inducere kontrolleret produktion af disse molekyler i celler og væv, der nøjagtigt ligner deres fysiologiske produktion som anden signalbudbringer. På nuværende tidspunkt involverer de fleste eksperimentelle tilgange administration af eksogen ROS, hovedsagelig i form af hydrogenperoxid. Vi har for nylig implementeret en eksperimentel tilgang til at tænde for en forbigående, ikke-dødelig in vivo-produktion af endogen ROS i musehuden baseret på administration af forstadier til det endogene fotosensibilisator protoporphyrin IX (PpIX; f.eks. aminolaevulinsyre eller dets methylderivat methylaminolevulinat) og yderligere bestråling af prøven med rødt lys for at inducere in situ-dannelse af ROS fra intracellulært molekylært ilt (figur 1). Denne fotodynamiske procedure kan effektivt anvendes til at stimulere residente stamcellenicher og dermed aktivere vævets regenerative programmer19,20 og åbne vejen for nye terapeutiske modaliteter inden for hudregenerativ medicin. Her præsenterer vi en detaljeret beskrivelse af protokollen, der viser repræsentative eksempler på stimulering af stamcellenicher, målt som en stigning i antallet af langsigtede 5-brom-2'-deoxyuridin (BrdU) etiketfastholdende celler (LRC'er) i udbulningsområdet i hårsækken19,21 og efterfølgende aktivering af regenereringsprogrammer (acceleration af hårvækst og brandhelingsprocesser) induceret af forbigående, ikke-dødelig ROS-produktion i huden på C57Bl6-musestammen.

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	<tbody>
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			<td height="21" style="height:21px;width:432px;">2&#8242;,7&#8242;-Dichlorofluorescin diacetate</td>
			<td style="width:104px;">Sigma Aldrich</td>
			<td style="width:115px;">D6883-50MG</td>
			<td style="width:161px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:432px;">5&#39;-bromo-2&#39;-deoxiuridine</td>
			<td style="width:104px;">Sigma Aldrich</td>
			<td style="width:115px;">B5002-500MG</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:432px;">Anti-Bromodeoxyuridine-Fluorescein</td>
			<td style="width:104px;">Roche</td>
			<td>11202693001</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Depilatory cream (e.g., Veet)</td>
			<td style="width:104px;">Veet</td>
			<td style="width:115px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:432px;">Dihydroethidium</td>
			<td style="width:104px;">Sigma Aldrich</td>
			<td>37291-25MG</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:432px;">In Vivo imaging system, e.g., IVIS Lumina 2</td>
			<td style="width:104px;">Perkin Elmer</td>
			<td style="width:115px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:432px;">mALA in the form of topical cream, e.g.,METVIX Crema 160 mg/g</td>
			<td style="width:104px;">Galderma</td>
			<td style="width:115px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:432px;">Power energy meter (e.g., ThorLabs Model PM100D)</td>
			<td style="width:104px;">ThorLabs</td>
			<td style="width:115px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:432px;">Red light source, e.g., 636 nm Aktilite LED lamp</td>
			<td style="width:104px;">Photocure ASA</td>
			<td style="width:115px;"></td>
			<td></td>
		</tr>
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stimulation of stem cell niches and tissue regeneration in mouse skin by switchable protoporphyrin ix-dependent photogeneration of reactive oxygen species in situ

Her beskriver vi en protokol til at inducere omskiftelig in vivo fotogenerering af endogene reaktive iltarter (ROS) i musehud. Denne forbigående produktion af ROS in situ aktiverer effektivt celleproliferation i stamcellenicher og stimulerer vævsregenerering som stærkt manifesteret gennem acceleration af brandheling og hårfollikelvækstprocesser. Protokollen er baseret på en regulerbar fotodynamisk behandling, der behandler vævet med forstadier til det endogene fotosensibilisator protoporphyrin IX og yderligere bestråler vævet med rødt lys under tæt kontrollerede fysisk-kemiske parametre. Samlet set udgør denne protokol et interessant eksperimentelt værktøj til at analysere ROS-biologi.

Topisk administration af mALA-prækursoren i musens ryg- og halehud resulterer i en signifikant ophobning af PpIX i hele vævet og mærkbart i hårsækken, som demonstreret ved den rødlig-lyserøde fluorescens af denne forbindelse under blåt lys (407 nm) excitation (figur 2A,C). Efterfølgende bestråling af behandlet væv med rødt lys (636 nm) ved en fluens på 2,5-4 J/cm2 fremmer forbigående produktion af ROS i vævet, især i hårsækkens buleområde (<stro...

Watch this Scientific Journal Video about Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In 

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Formålet med denne protokol er at fremkalde forbigående in vivo-produktion af ikke-dødelige niveauer af reaktive iltarter (ROS) i musehud, hvilket yderligere fremmer fysiologiske reaktioner i vævet.

Stimulering af stamcellenicher og vævsregenerering i musehud ved omskiftelig protoporphyrin IX-afhængig fotogenerering af reaktive iltarter in situ

Here, we describe a protocol to induce switchable in vivo photogeneration of endogenous reactive oxygen species (ROS) in mouse skin. This transient ...

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