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DOI :
10.3791/60927-v
•
10:00 min
March 4th, 2020
Chapters
0:05
Introduction
0:56
Preparation of Solutions
1:57
Preparation of Tissue
2:49
Homogenization of Sample
4:03
Equilibration of Beads
4:59
Preclearing Sample
5:56
Incubation of Antibody and Beads
6:29
Washing of Beads
7:21
RNA Extraction
7:54
Results: Negative Controls, Reagents Impact, and a Mutant Model
9:09
Conclusion
在这里,我们描述了核糖体和相关RNA的免疫沉淀从成年雄性小鼠生殖细胞的精选群体使用RiboTag系统。战略性育种和精心的免疫沉淀产生清洁、可重复的结果,为生殖细胞的翻译提供信息,并深入了解突变表型的机制。
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