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DOI :
10.3791/60927-v
•
10:00 min
March 4th, 2020
Chapters
0:05
Introduction
0:56
Preparation of Solutions
1:57
Preparation of Tissue
2:49
Homogenization of Sample
4:03
Equilibration of Beads
4:59
Preclearing Sample
5:56
Incubation of Antibody and Beads
6:29
Washing of Beads
7:21
RNA Extraction
7:54
Results: Negative Controls, Reagents Impact, and a Mutant Model
9:09
Conclusion
ここでは、リボタグシステムを用いた成人男性マウス生殖細胞の選択集団からリボソームおよび関連RNAの免疫沈降について説明する。戦略的な繁殖と慎重な免疫沈降は、細菌細胞の翻訳を知らせ、変異型のメカニズムに関する洞察を提供するクリーンで再現可能な結果をもたらします。
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