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DOI :
10.3791/60939-v
April 12th, 2020
Chapters
0:05
Introduction
1:17
Animal Perfusion and Brain Preservation
2:01
Glioma Brain Tumors Tissue Cryopreservation
2:52
Frozen Brain Tumor Tissue Sectioning
3:58
Cryopreserved Brain Tissue Section Fixation and Staining
4:48
Laser Capture Microdissection
6:30
Results: Representative RNA Quality Control and Library Preparation
7:55
Conclusion
レーザーマイクロディション(LMD)は、グリオーマの不均一性と侵入を媒介する経路を明らかにするために使用できる、敏感で再現性の高い技術です。ここでは、レーザーLMDを用いて、トランスクリプトーム解析を用いて、グリオーマ組織から離散領域を分離するための最適化されたプロトコルについて述べている。
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