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DOI :
10.3791/60960-v
•
10:02 min
April 29th, 2020
Chapters
0:04
Introduction
0:42
Embyroid Body Generation
2:51
Dissociation of EBs
3:48
Fixation
4:21
Cell Lysis
5:03
Restriction Enzyme Digestion
6:34
DNA Shearing and Size Selection
7:31
Results: Enhance-Promoter Contacts During EB Differentiation
9:13
Conclusion
我々は、胚性幹細胞から生成された胚体における高スループットシーケンシングに続く定量染色体立体構造捕捉の適用を報告する。この技術は、胚性幹細胞分化中に所定の遺伝子の推定エンハンサーとプロモーター領域との接触を同定し、定量することを可能にする。
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