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DOI :
10.3791/60970-v
•
7:44 min
June 8th, 2020
Chapters
0:04
Introduction
0:31
Protein Extraction
1:40
Reducing/Alkylating Reagent Treatment
2:39
Methanol/Chloroform Precipitation
4:20
Protein Digestion
4:56
Peptide Labeling
5:57
Results: Representative Protein Quantification and Volcano Plot and Protein Analysis through Evolutionary Relationships (PANTHER) Analyses
7:04
Conclusion
我们提出了优化的串联质量标签 (TMT) 标签协议,其中包括以下每个步骤的详细信息:蛋白质提取、定量、沉淀、消化、标记、提交到蛋白质组学设施以及数据分析。
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