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DOI :
10.3791/61008-v
April 24th, 2020
Chapters
0:04
Introduction
0:39
Spleen, Contralateral Bone Marrow, Ipsilateral Lymph Nodes, and Synovial Tissue Isolation
4:18
Tissue Processing: Spleen
5:16
Tissue Processing: Lymph Node and Bone Marrow
6:42
Tissue Processing: Synovial Tissue
7:57
Monocyte and T cell Subset Panel Immunostaining
10:28
Results: Representative Mouse Osteoarthritis Model Immune Cell Subset Analysis
11:45
Conclusion
여기에서, 우리는 뮤린 비장, 골수, 림프절 및 교압 조직 내의 엑스트라 세포 염색 분석체를 모두 사용하여 단세포/대식세포 및 T 세포 하위 세트를 식별하기 위해 상세하고 재현 가능한 유동 세포 측정 프로토콜을 설명하며, 골관절염의 확립된 수술 모델을 활용합니다.
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