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6.7K Views
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07:23 min
May 5th, 2020
DOI :
10.3791/61062-v
Chapters
0:04
Introduction
0:51
Chemical of Interest Preparation and In Situ Chemotaxis Assay (ISCA) Filling for Lab Deployment
1:49
Lab Deployment
2:37
Laboratory Sample Retrieval
3:09
Field Chemoattractant Preparation
4:12
Field Deployment
5:55
Results: Representative In Vitro and Field Microbial Chemotaxis Analyses
6:41
Conclusion
Transcript
我们的协议使我们能够在它们的家中直接对微生物化学进行可靠的量化。因此,我们可以分离微生物,我们可以更好地量化和理解代谢潜力。原位化学测定,简言之,ISCA是一种灵活、用户友好和简单的设备,能够对微生物行为有新的洞察,从而发现微生物在任何具有液相的环境中的作用和功能。
Estelle Clerc 是实验室和现场部署程序,他目前是我的研究小组的博士生。在准备化学分析器之前,使用 0.2 微米过滤器过滤介质,然后自动解过滤过滤溶液。接下来,在无菌介质的1毫升中准备10毫升的化疗剂溶液
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Summary
这里介绍的是原位化疗的规程,这是一种最近开发的微流体装置,能够直接研究环境中的微生物行为。
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