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DOI :
10.3791/61090-v
•
9:27 min
May 6th, 2020
Chapters
0:04
Introduction
1:11
Peripheral Blood Monocytes (PBM) Isolation
3:07
CD14+ Cell Selection
4:00
PBM Seeding and Differentiation
4:46
Human Alveolar Epithelial Tissue Triple Cell Coculture Model
5:22
MDDC and MDM Seeding
7:03
Coculture Model Transfer to Air-Liquid Interface
7:30
Results: Representative Multicellular Human Alveolar Model Hazard Assessment
8:52
Conclusion
这里介绍的是原发性人类血液单细胞分离的协议,以及它们分化成巨噬细胞和树突状细胞,并组装上皮细胞成多细胞人类肺模型。比较了由免疫细胞组成的生物反应,这些细胞与新鲜分离或解冻的单核细胞不同,在接触刺激性刺激时。
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