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DOI :
10.3791/61110-v
May 21st, 2020
Chapters
0:04
Introduction
0:43
Surgical Preparation
1:39
Pregnant Mouse Preparation
2:16
Intraventricular Injection
3:16
In Utero Electroporation (IUE)
4:24
Multichannel Confocal Imaging
5:23
Results: Representative Cortical Astrocyte Segmentation and Arborization
6:10
Conclusion
アストロサイトは大脳皮質を均一にタイル化し、複雑な形態の分析を細胞レベルで困難にします。ここで提供されるプロトコルは、子宮内のエレクトロポレーションに基づく多色標識を使用して、皮質アストロサイトを単一にし、ユーザーフレンドリーな画像解析パイプラインでボリュームと形態を分析します。
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