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DOI :
10.3791/61127-v
May 16th, 2020
Chapters
0:05
Introduction
0:45
Primary Fibroblast Isolation and Culture
3:04
Purification of Exosomes Using Differential and Ultra-Centrifugation
4:58
Results: Characterization of Purified Exosomes
5:47
Conclusion
该方案说明了1)从成年小鼠腓肠肌中分离和培养原代成纤维细胞,以及2)使用差异超速离心法结合蔗糖密度梯度,然后进行蛋白质印迹分析的外泌体纯化和表征。
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