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DOI :
10.3791/61127-v
May 16th, 2020
Chapters
0:05
Introduction
0:45
Primary Fibroblast Isolation and Culture
3:04
Purification of Exosomes Using Differential and Ultra-Centrifugation
4:58
Results: Characterization of Purified Exosomes
5:47
Conclusion
このプロトコルは1)大人マウス腓腹筋からの第一次線維芽細胞の隔離そして文化、また2)西部のしみの分析に先行しているスクロース密度勾配と結合される差動ultrarifjugeation方法を使用してexosomesの浄化そして性格描写を示す。
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