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DOI :
10.3791/61127-v
May 16th, 2020
Chapters
0:05
Introduction
0:45
Primary Fibroblast Isolation and Culture
3:04
Purification of Exosomes Using Differential and Ultra-Centrifugation
4:58
Results: Characterization of Purified Exosomes
5:47
Conclusion
이 프로토콜은 1) 성체 마우스 비복근에서 일차 섬유아세포를 분리하고 배양하는 방법과 2) 자당 밀도 구배와 웨스턴 블롯 분석을 결합한 차등 초원심분리 방법을 사용하여 엑소좀을 정제하고 특성화하는 방법을 보여줍니다.
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