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DOI :
10.3791/61138-v
June 10th, 2020
Chapters
0:04
Introduction
0:44
In Vivo Ubiquitylation Assays Using pQCXIP-EYFP-CENP-A
2:19
Mass Spectrometry to Identify the Ubiquitylation Site of the EYFP-CENP-A K124R Mutant
4:55
Data Analysis
6:22
Results: EYFP-CENP-A K124 Mutant is Ubiquitylated and Interacts with HJURP
8:12
Conclusion
CENP-A泛化是CENP-A在中心区沉积的重要要求,通过细胞分裂之间的二化遗传,对细胞的生存能力不可或缺。在这里,我们描述质谱分析,以确定EYFP标记CENP-A(EYFP-CENP-A)蛋白的泛化。
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