Name: synthesis of aptamer-pei-g-peg modified gold nanoparticles loaded with doxorubicin for targeted drug delivery
Uploaded: 2020-06-23T13:30:00
Description: Watch this Scientific Journal Video about Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery at JoVE.com

Denne forskningen ble finansiert av National Natural Science Foundation of China (31700840); Det sentrale vitenskapelige forskningsprosjektet i Henan-provinsen (18B430013, 18A150049). Denne forskningen ble støttet av Nanhu Scholars Program for Young Scholars of XYNU. Forfatterne vil takke bachelorstudenten Zebo Qu fra College of Life Sciences i XYNU for hans nyttige verk. Forfatterne ønsker å anerkjenne Analyse - testsenteret til XYNU for bruk av utstyret deres.

FORSIKTIG: Sørg for å konsultere alle relevante sikkerhetsdatablad (MSDS) før du bruker alle kjemikalier. Flere av kjemikaliene som brukes til å forberede kopolymer og nanopartikler er akutt giftige. Nanopartikler har også potensielle farer. Sørg for å bruke alle passende sikkerhetsrutiner og personlig verneutstyr, inkludert hansker, labfrakk, hetter, bukser i full lengde og sko i nærheten.
1. Syntese av dobbelkarboksylpolyetylenglykol (CT-PEG)33
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	<li>Abad, J. M., Bravo, I., Pariente, F., Lorenzo, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multi-tasking+base+ligand:+a+new+concept+of+AuNPs+synthesis.">Multi-tasking base ligand: a new concept of AuNPs synthesis.</a> Analytical and Bioanalytical Chemistry. 408 (9), 2329-2338 (2016).</li><li>Siegel, R. L., Miller, K. D., Jemal, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cancer+statistics,+2019.">Cancer statistics, 2019.</a> CA-A Cancer Journal for Clinicians. 69 (1), 7-34 (2019).</li><li>Jang, B., Kwon, H., Katila, P., Lee, S. 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S., Murphy, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recent+Progress+in+Cancer+Thermal+Therapy+Using+Gold+Nanoparticles.">Recent Progress in Cancer Thermal Therapy Using Gold Nanoparticles.</a> The Journal of Physical Chemistry C. 120 (9), 4691-4716 (2016).</li><li>Riley, R. S., Day, E. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gold+nanoparticle-mediated+photothermal+therapy:+applications+and+opportunities+for+multimodal+cancer+treatment.">Gold nanoparticle-mediated photothermal therapy: applications and opportunities for multimodal cancer treatment.</a> WIREs Nanomedicine and Nanobiotechnology. 9 (4), 1449 (2017).</li><li>Fratoddi, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Highly+Hydrophilic+Gold+Nanoparticles+as+Carrier+for+Anticancer+Copper(I)+Complexes:+Loading+and+Release+Studies+for+Biomedical+Applications.">Highly Hydrophilic Gold Nanoparticles as Carrier for Anticancer Copper(I) Complexes: Loading and Release Studies for Biomedical Applications.</a> Nanomaterials (Basel). 9 (5), 772 (2019).</li><li>Lee, S. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drug-loaded+gold+plasmonic+nanoparticles+for+treatment+of+multidrug+resistance+in+cancer.">Drug-loaded gold plasmonic nanoparticles for treatment of multidrug resistance in cancer.</a> Biomaterials. 35 (7), 2272-2282 (2014).</li><li>Dreaden, E. C., Alkilany, A. M., Huang, X., Murphy, C. J., El-Sayed, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+golden+age:+gold+nanoparticles+for+biomedicine.">The golden age: gold nanoparticles for biomedicine.</a> Chemical Society Reviews. 41 (7), 2740-2779 (2012).</li><li>Wei, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anticancer+drug+nanomicelles+formed+by+self-assembling+amphiphilic+dendrimer+to+combat+cancer+drug+resistance.">Anticancer drug nanomicelles formed by self-assembling amphiphilic dendrimer to combat cancer drug resistance.</a> Proceedings of the National Academy of Sciences of the United States of America. 112 (10), 2978-2983 (2015).</li><li>Galluzzi, L., Buque, A., Kepp, O., Zitvogel, L., Kroemer, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Immunological+Effects+of+Conventional+Chemotherapy+and+Targeted+Anticancer+Agents.">Immunological Effects of Conventional Chemotherapy and Targeted Anticancer Agents.</a> Cancer Cell. 28 (6), 690-714 (2015).</li><li>Muddineti, O. S., Ghosh, B., Biswas, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current+trends+in+using+polymer+coated+gold+nanoparticles+for+cancer+therapy.">Current trends in using polymer coated gold nanoparticles for cancer therapy.</a> International Journal of Pharmaceutics. 484 (1-2), 252-267 (2015).</li><li>Hu, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methyl+Orange+removal+by+a+novel+PEI-AuNPs-hemin+nanocomposite.">Methyl Orange removal by a novel PEI-AuNPs-hemin nanocomposite.</a> Journal of Environmental Sciences. 53, 278-283 (2017).</li><li>Gu, F. X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeted+nanoparticles+for+cancer+therapy.">Targeted nanoparticles for cancer therapy.</a> Nano Today. 2 (3), 14-21 (2007).</li><li>Srinivasarao, M., Galliford, C. V., Low, P. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Principles+in+the+design+of+ligand-targeted+cancer+therapeutics+and+imaging+agents.">Principles in the design of ligand-targeted cancer therapeutics and imaging agents.</a> Nature Reviews Drug Discovery. 14 (3), 203-219 (2015).</li><li>Liu, Z., Shi, Y., Chen, Z., Duan, L., Wang, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current+progress+towards+the+use+of+aptamers+in+targeted+cancer+therapy.">Current progress towards the use of aptamers in targeted cancer therapy.</a> Chinese Science Bulletin (Chinese Version). 59 (14), 1267 (2014).</li><li>Ghosh, P., Han, G., De, M., Kim, C. K., Rotello, V. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gold+nanoparticles+in+delivery+applications.">Gold nanoparticles in delivery applications.</a> Advanced Drug Delivery Reviews. 60 (11), 1307-1315 (2008).</li><li>Vandghanooni, S., Eskandani, M., Barar, J., Omidi, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antisense+LNA-loaded+nanoparticles+of+star-shaped+glucose-core+PCL-PEG+copolymer+for+enhanced+inhibition+of+oncomiR-214+and+nucleolin-mediated+therapy+of+cisplatin-resistant+ovarian+cancer+cells.">Antisense LNA-loaded nanoparticles of star-shaped glucose-core PCL-PEG copolymer for enhanced inhibition of oncomiR-214 and nucleolin-mediated therapy of cisplatin-resistant ovarian cancer cells.</a> International Journal of Pharmaceutics. 573, 118729 (2020).</li><li>Andghanooni, S., Eskandani, M., Barar, J., Omidi, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=AS1411+aptamer-decorated+cisplatin-loaded+poly(lactic-co-glycolic+acid)+nanoparticles+for+targeted+therapy+of+miR-21-inhibited+ovarian+cancer+cells.">AS1411 aptamer-decorated cisplatin-loaded poly(lactic-co-glycolic acid) nanoparticles for targeted therapy of miR-21-inhibited ovarian cancer cells.</a> Nanomedicine. 13 (21), 2729-2758 (2018).</li><li>Palmieri, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+anti-nucleolin+recombinant+immunoagent+for+cancer+therapy.">Human anti-nucleolin recombinant immunoagent for cancer therapy.</a> Proceedings of the National Academy of Sciences of the United States of America. 112 (30), 9418-9423 (2015).</li><li>Pichiorri, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+NCL+targeting+affects+breast+cancer+aggressiveness+through+miRNA+regulation.">In vivo NCL targeting affects breast cancer aggressiveness through miRNA regulation.</a> Journal of Experimental Medicine. 210 (5), 951-968 (2013).</li><li>Hou, S., McCauley, L. K., Ma, P. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synthesis+and+erosion+properties+of+PEG-containing+polyanhydrides.">Synthesis and erosion properties of PEG-containing polyanhydrides.</a> Macromolecular Bioscience. 7 (5), 620-628 (2007).</li><li>Nie, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Injectable+Vaginal+Hydrogels+as+a+Multi-Drug+Carrier+for+Contraception.">Injectable Vaginal Hydrogels as a Multi-Drug Carrier for Contraception.</a> Applied Sciences. 9 (8), 1638 (2019).</li><li>Zou, P., Suo, J., Nie, L., Feng, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Temperature-responsive+biodegradable+star-shaped+block+copolymers+for+vaginal+gels.">Temperature-responsive biodegradable star-shaped block copolymers for vaginal gels.</a> Journal of Materials Chemistry. 22 (13), 6316-6326 (2012).</li><li>Etrych, T., &#352;ubr, V., Laga, R., &#344;&#237;hov&#225;, B., Ulbrich, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polymer+conjugates+of+doxorubicin+bound+through+an+amide+and+hydrazone+bond:+Impact+of+the+carrier+structure+onto+synergistic+action+in+the+treatment+of+solid+tumours.">Polymer conjugates of doxorubicin bound through an amide and hydrazone bond: Impact of the carrier structure onto synergistic action in the treatment of solid tumours.</a> European Journal of Pharmaceutical Sciences. 58, 1-12 (2014).</li><li>Safari, F., Tamaddon, A. 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1H NMR-spekteret (figur 2) bekrefter den vellykkede syntesen av CT-PEG-kopulering og PEI-g-PEG-kokopolymer. Molekylvektene til PEG og PEI var henholdsvis 1000 og 1200. I tillegg ble det EDC/NHS katalytiske systemet brukt til å syntetisere PEI-g-PEG-kopulering via midtreaksjoner. Det skal bemerkes at hvis molekylvektene til PEG og PEI endret seg for å syntetisere PEI-g-PEG-kopulering, må reaksjonstiden og katalytisk system revurderes. Også reaksjonsbetingelsen for PEI-g-PEG-kop...

Å være det største folkehelseproblemet over hele verden, er kreft allment karakterisert som å ha en lav kurrate, høy tilbakefallsrate og høydødelighet 1,2. Nåværende konvensjonelle anti-kreft metoder inkluderer kirurgi, kjemoterapi ogstrålebehandling 3, blant annet kjemoterapi er den primære behandlingen for kreftpasienter i klinikken4. Kliniske brukte anticancer medisiner inkluderer hovedsakelig paclitaxel (PTX)5 og doxorubicin (DOX)6,7. DOX, et antineoplastisk legemiddel, har blitt bredt brukt i klinisk kjemoterapi, på grunn av fordelene med kreft cytotoksisitet og hemming av kreftcelleproliferasjon8,9. DOX forårsaker imidlertid kardiotoksisitet10,11, og den korte halveringstiden til DOX begrenser anvendelsen i klinikken12. Derfor er nedbrytbare legemiddelbærere nødvendig for å laste DOX og subtilt frigjøre på en kontrollert måte til et målrettet område.
Nanopartikler har blitt mye brukt i målrettede legemiddelleveringssystemer og har flere fordeler ved kreftbehandling (dvs. stort overflate-til-volum-forhold, liten størrelse, evne til å innkapsle ulike stoffer og justerbar overflatekjemi, etc.) 13,14,15. Spesielt har gull nanopartikler (AuNPer) blitt mye brukt i biologiske og biomedisinske applikasjoner, for eksempel fototermisk kreftbehandling16,17. De unike egenskapene til AuNPer, som facile syntese og generell overflatefunksjonalisering, har gode utsikter innen det kliniske feltet kreftbehandling18. Også AuNPer har blitt brukt til å identifisere narkotika levering strategier, diagnostisere svulster, og overvinne motstand i mange studier19,20.
Til tross for dette må AuNPer skreddersys ytterligere for å overvinne legemiddelresistens via høy lokal frigjøring ved tumorlesjoner gjennom forbedret permeasjon og oppbevaring (EPR), for eksempel målretting og tilgjengelighetsegenskaper. Polymerfunksjonaliserte AuNPer har vist unike fordeler, for eksempel forbedret vannløselighet av hydrofobe anticancer medisiner og langvarigsirkulasjonstid 21,22. Ulike biokompatible polymerer har blitt brukt til AuNP-belegg, for eksempel polyetylenglykol (PEG), polyetylenimin (PEI), hyaluronsyre, heparin og xanthan tyggegummi. Da er stabiliteten, så vel som nyttelasten, av AuNPer forbedret godt23. Spesielt er PEI en svært forgrenet polymer som består av mange repeterende enheter av primære, sekundære og tertiære aminer24. PEI har utmerket løselighet, lav viskositet og en høy grad av funksjonalitet, som er egnet for belegg på AuNPer.
På den annen side må antikreftmedisiner leveres til kreftceller direkte med forbedret lasteeffektivitet, og med lavere toksisitet for behandling av primære og avanserte metastatiske svulster25. Målrettede ligander har et stort potensial for anti-kreft narkotika målrettet levering systemer26. Dens selektivitet for målmolekylbinding gir anti-kreftmedisin rettet mot spesifisitet og øker legemiddelberikelse i syke vev27. Flere ligander inkluderer antistoffer, polypeptider og små molekyler. Sammenlignet med andre ligander, kan nukleinsyreaptamerer syntetiseres in vitro og er enkle å modifisere. AS1411 er en umodifisert 26 bp fosfodiester oligonukleotid som danner en stabil dimerisk G-tetramerstruktur for å spesifikt binde seg til en overekspressert mål kjernefysisk proteinreseptor på kreftceller28,29,30. AS1411 hemmer spredningen av mange kreftceller, men påvirker ikke veksten av sunne celler31,32. Som et resultat har AS1411 blitt brukt til å fremstille et ideelt målrettet legemiddelleveringssystem.
I denne studien syntetiseres en PEI-g-PEG-kopolymer via en midtreaksjon, deretter er PEI-g-PEG copolymerbelagte gull nanopartikler (PEI-g-PEG@AuNPs) fremstilt. I tillegg er DOX og AS1411 sekvensielt knyttet til de forberedte PEI-g-PEG@AuNPs, som vist i figur 1. Denne detaljerte protokollen er ment å hjelpe forskere med å unngå mange av de vanlige fallgruvene forbundet med fabrikasjon av nye PEI-g-PEG@AuNPs lastet med DOX og AS1411.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>4-Dimethylaminopyridine</td>
			<td>Macklin</td>
			<td>D807273</td>
			<td></td>
		</tr>
		<tr>
			<td>A549 cell</td>
			<td></td>
			<td>ATCC CCL-185TM</td>
			<td></td>
		</tr>
		<tr>
			<td>AS1411</td>
			<td>BBI Life Sciences Corporation</td>
			<td></td>
			<td>5&#39;-d (TTTGGTGGTGGTGGTTGTGGTGGTGGTGG) FL-AS1411 (fluorophore-labeled AS1411)</td>
		</tr>
		<tr>
			<td>Anhydrous Tetrahydrofuran (THF)</td>
			<td>SinoPharm Chemical Reagent Co., Ltd</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Cell counting kit-8 (CCK-8)</td>
			<td>Sigma Aldrich</td>
			<td>96992-500TESTS-F</td>
			<td></td>
		</tr>
		<tr>
			<td>Dichloromethane</td>
			<td>Traditional Chinese medicine</td>
			<td>80047318</td>
			<td></td>
		</tr>
		<tr>
			<td>Diethyl ether (Et2O)</td>
			<td>SinoPharm Chemical Reagent Co., Ltd</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Dimethyl sulfoxide</td>
			<td>Macklin</td>
			<td>D806645</td>
			<td></td>
		</tr>
		<tr>
			<td>Dulbecco&#39;s modified Eagle&#39;s medium (DMEM)</td>
			<td>Sigma Aldrich</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Doxorubicin hydrochloride</td>
			<td>Rhawn</td>
			<td>R017518</td>
			<td></td>
		</tr>
		<tr>
			<td>Ether absolute</td>
			<td>Traditional Chinese medicine</td>
			<td>80059618</td>
			<td></td>
		</tr>
		<tr>
			<td>Field Emission Transmission Electron Microscope</td>
			<td>FEI Company</td>
			<td>Tecnai G2 F 20</td>
			<td></td>
		</tr>
		<tr>
			<td>Gold(III) chloride trihydrate</td>
			<td>Rhawn</td>
			<td>R016035</td>
			<td></td>
		</tr>
		<tr>
			<td>Laser Particle-size Instrument</td>
			<td>Malvern Instruments Ltd</td>
			<td>ZetasizerNanoZS/Masterszer3000E</td>
			<td></td>
		</tr>
		<tr>
			<td>Microplate Reader</td>
			<td>Molecular Devices</td>
			<td>SpectraMax 190</td>
			<td></td>
		</tr>
		<tr>
			<td>N-(3-Dimethylaminopropyl)-N&#39;-ethylcarbodiimide hydrochloride</td>
			<td>Macklin</td>
			<td>N808856</td>
			<td></td>
		</tr>
		<tr>
			<td>N-Hydroxysuccinimide</td>
			<td>Macklin</td>
			<td>H6231</td>
			<td></td>
		</tr>
		<tr>
			<td>NMR software</td>
			<td>Delta 5.2.1</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Nuclear Magnetic Resonance Spectrometer</td>
			<td>JEOL</td>
			<td>JNM-ECZ600R/S3</td>
			<td></td>
		</tr>
		<tr>
			<td>Origin 8.5</td>
			<td>OriginLab</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Penicillin</td>
			<td>Sigma Aldrich</td>
			<td>V900929-100ML</td>
			<td></td>
		</tr>
		<tr>
			<td>Phosphate-buffered saline</td>
			<td>Sigma Aldrich</td>
			<td>P4417-100TAB</td>
			<td></td>
		</tr>
		<tr>
			<td>Poly(ethylene glycol)</td>
			<td>Sigma Aldrich</td>
			<td>81188</td>
			<td>BioUltra, average Mn ~ 1000</td>
		</tr>
		<tr>
			<td>Poly (ethyleneimine) solution</td>
			<td>Sigma Aldrich</td>
			<td>482595</td>
			<td>average Mn ~ 1200, 50 wt.% in H2O</td>
		</tr>
		<tr>
			<td>Sodium borohydride, powder</td>
			<td>Acros</td>
			<td>C18930</td>
			<td></td>
		</tr>
		<tr>
			<td>Streptomycin</td>
			<td>Sigma Aldrich</td>
			<td>85886-10ML</td>
			<td></td>
		</tr>
		<tr>
			<td>Succinic anhydride</td>
			<td>Traditional Chinese medicine</td>
			<td>30171826</td>
			<td></td>
		</tr>
		<tr>
			<td>Tetrahydrofuran</td>
			<td>Traditional Chinese medicine</td>
			<td>40058161</td>
			<td></td>
		</tr>
		<tr>
			<td>Triethylamine</td>
			<td>Traditional Chinese medicine</td>
			<td>80134318</td>
			<td></td>
		</tr>
		<tr>
			<td>UV/VIS/NIR Spectrometer</td>
			<td>Lambda950</td>
			<td>Lambda950</td>
			<td></td>
		</tr>
		<tr>
			<td>X-ray Photoelectron Spectrometer</td>
			<td>Thermo&#8194;Fisher&#8194;Scientific</td>
			<td>K-ALPHA 0.5EV</td>
			<td></td>
		</tr>
	</tbody>
</table>

synthesis of aptamer-pei-g-peg modified gold nanoparticles loaded with doxorubicin for targeted drug delivery

På grunn av legemiddelresistens og toksisitet i friske celler har bruk av doksorubicin (DOX) vært begrenset i klinisk kreftbehandling. Denne protokollen beskriver utformingen av poly(etylenimine) podet med polyetylenglykol (PEI-g-PEG) kopolymerfunksjonaliserte gull nanopartikler (AuNPer) med lastet aptamer (AS1411) og DOX gjennom midtreaksjoner. AS1411 er spesielt knyttet til målrettede nukleolinreseptorer på kreftceller, slik at DOX retter seg mot kreftceller i stedet for friske celler. Først blir PEG karboksylert, deretter podet til forgrenet PEI for å få en PEI-g-PEG-kopulering, som bekreftes av 1H NMR-analyse. Deretter syntetiseres PEI-g-PEG copolymer-belagte nanopartikler av gull (PEI-g-PEG@AuNPs), og DOX og AS1411 knyttes gradvis til AuNPer via midtreaksjoner. Diameteren på den tilberedte AS1411-g-DOX-g-PEI-g-PEG@AuNPs er ~ 39,9 nm, med et zetapotensial på -29,3 mV, noe som indikerer at nanopartiklene er stabile i vann og cellemedium. Cellecytoksisitetsanalyser viser at de nydesignede DOX-lastede AuNP-ene er i stand til å drepe kreftceller (A549). Denne syntesen demonstrerer det delikate arrangementet av PEI-g-PEG-kopolymerer, aptamers og DOX på AuNPer som oppnås ved sekvensielle midtreaksjoner. Slike aptamer-PEI-g-PEG-funksjonaliserte AuNPer gir en lovende plattform for målrettet legemiddellevering i kreftbehandling.

1 H NMR-spektroskopi ble brukt til å bekrefte vellykket syntese av CT-PEG-polymer og PEI-g-PEG-kopolymerer (figur 2). Figur 2a viser at metylenprotonsignalet ved δ = 3,61 ppm og karboksylprotonsignal ved δ = 2,57 ppm bekrefter vellykket syntese av CT-PEG polymerer. Figur 2b viser at metylenprotonsignalet til PEG ved δ = 2,6 ppm og protonsignal for PEI ved δ = 1,66 ppm bekrefter syn...

Watch this Scientific Journal Video about Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery at JoVE.com

Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery

I denne protokollen syntetiseres doksorubicinbelastede AS1411-g-PEI-g-PEG-modifiserte gull nanopartikler via tre-trinns midt i reaksjoner. Deretter lastes doksorubicin og leveres for å målrette kreftceller for kreftbehandling.

Syntese av Aptamer-PEI-g-PEG Modifisert Gull Nanopartikler Lastet med Doxorubicin for målrettet legemiddellevering

Due to drug resistance and toxicity in healthy cells, use of doxorubicin (DOX) has been limited in clinical cancer therapy. This protocol describes the ...

Research

JoVE Journal

Chemistry

Template Directed Synthesis of Plasmonic Gold Nanotubes with Tunable IR Absorbance

Mal Regissert Syntese av Plasmonic Gold Nanorør med Fleksibel IR Absorbans

A nearly parallel array of pores can be produced by anodizing aluminum foils in acidic environments1, 2. Applications of anodic aluminum oxide (AAO) ...

Preparation and Characterization of SDF-1&#945;-Chitosan-Dextran Sulfate Nanoparticles

Preparation and Characterization of SDF-1ÃƒÅ½Ã‚Â±-Chitosan-Dextran Sulfate Nanoparticles

Utarbeidelse og karakterisering av SDF-1α-Chitosan-dekstransulfat Nanopartikler

Chitosan (CS) and dextran sulfate (DS) are charged polysaccharides (glycans), which form polyelectrolyte complex-based nanoparticles when mixed under ...

A Simple Method for the Size Controlled Synthesis of Stable Oligomeric Clusters of Gold Nanoparticles under Ambient Conditions

En enkel metode for størrelsen Kontrollert Syntese av Stabile Oligomere Klynger av gull nanopartikler under omgivelses

Reducing dilute aqueous HAuCl4 with sodium thiocyanate (NaSCN) under alkaline conditions produces 2 to 3 nm diameter nanoparticles. Stable grape-like ...

Protocol for the Synthesis of Ortho-trifluoromethoxylated Aniline Derivatives

Protocol for the Synthesis of Ortho-trifluoromethoxylated Aniline Derivatives

Protokoll for syntese av<em&gt; Ortho</em&gt; -trifluoromethoxylated anilinderivater

Molecules bearing trifluoromethoxy (OCF3) group often show desired pharmacological and biological properties. However, facile synthesis of ...

Hydroquinone Based Synthesis of Gold Nanorods

Hydrokinon Basert Syntese av gull nanorods

Gold nanorods are an important kind of nanoparticles characterized by peculiar plasmonic properties. Despite their widespread use in nanotechnology, the ...

Chemical Precipitation Method for the Synthesis of Nb2O5 Modified Bulk Nickel Catalysts with High Specific Surface Area

Chemical Precipitation Method for the Synthesis of Nb2O5 Modified Bulk Nickel Catalysts with High Specific Surface Area

Kjemisk nedbør metoden for syntese av Nb2O5 endret Bulk nikkel katalysatorer med høy bestemt areal

We demonstrate a method for the synthesis of NixNb1-xO catalysts with sponge-like and fold-like nanostructures. By varying the Nb:Ni ratio, a series of ...

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rask Nanoprobe Signal forsterkning av i Situ gull hydrogenion syntese

The use of nanoprobes such as gold, silver, silica or iron-oxide nanoparticles as detection reagents in bioanalytical assays can enable high sensitivity ...

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides

Syntese og karakterisering av 1,2-Dithiolane endret selv av peptider

This report focuses on the synthesis of an N-terminus 1,2-dithiolane modified self-assembling peptide and the characterization of the resulting ...

Molten-Salt Synthesis of Complex Metal Oxide Nanoparticles

Smeltet Salt syntese av komplekse metalloksid nanopartikler

The development of feasible synthesis methods is critical for the successful exploration of novel properties and potential applications of nanomaterials. ...

Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery

Metionin Funksjonalisert biokompatible Block Kopolymerer for målrettet plasmider DNA levering

Reversible addition-fragmentation chain transfer (RAFT) polymerization integrates the advantages of radical polymerization and living polymerization. This ...