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DOI :
10.3791/61152-v
•
9:03 min
May 10th, 2020
Chapters
0:05
Introduction
0:40
Gene Editing of Human Pluripotent Stem Cells
6:57
Results: Genetic Editing in Human Pluripotent Stem Cells
8:06
Conclusion
在这里,我们描述了一种使用基于 piggyBac的供体质粒和Cas9切口酶突变体在人类多能干细胞中进行无缝基因编辑的详细方法。将两点突变引入人胚胎干细胞(hESCs)肝细胞核因子4α(HNF4α)位点的外显子8。
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