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DOI :
10.3791/61156-v
May 12th, 2020
Chapters
0:05
Introduction
0:35
Preparation of the Corneoscleral Button
2:45
Maintenance of the Corneoscleral Buttons
3:29
Preparation of an Inoculum
4:17
Infecting the Corneoscleral Button
5:54
Homogenization of the Cornea
7:21
Results: Ex Vivo Model Mimics In Vivo Infection
8:35
Conclusion
本文描述了建立细菌性角膜炎前活体猪模型的分步协议。 伪多莫纳斯阿鲁吉诺萨 被用作原型生物体。这种创新模式模仿体内感染,因为细菌的增殖取决于细菌破坏角膜组织的能力。
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