Name: evaluation of left ventricular structure and function using 3d echocardiography
Uploaded: 2020-10-28T12:30:00
Description: Watch this Scientific Journal Video about Evaluation of Left Ventricular Structure and Function using 3D Echocardiography at JoVE.com

Projektnr. NVKP_16-1-2016-0017 ("Nationalt hjerteprogram") er blevet gennemført med støtte fra Ungarns nationale forsknings-, udviklings- og innovationsfond, finansieret under NVKP_16 finansieringsordning. Forskningen blev finansieret af Tema excellence-programmet (2020-4.1.1.-TKP2020) under ministeriet for innovation og teknologi i Ungarn inden for rammerne af Semmelweis-universitetets terapeutiske udviklings- og bioimaging-tematiske programmer.

Denne protokol følger retningslinjerne fra Semmelweis University Regional and Institutional Committee of Science and Research Ethics. Den nuværende protokol gælder for en bestemt kreditor. Selvom nogle trin forbliver gyldige uanset ultralydsmaskinen og efterbehandlingssoftwaren, kan der være vigtige forskelle, hvis du bruger andre leverandørers løsninger.
1. Tekniske krav
<ol><li>Udnyt en ekkokardiografi maskine i stand til 3D-billeddannelse.</li>
	<li>Tilslut en 3D transthoracic ekkok...

<ol>
	<li>Guta, A. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+echocardiography+to+assess+left+ventricular+geometry+and+function.">Three-dimensional echocardiography to assess left ventricular geometry and function.</a> Expert Review of Cardiovascular Therapy. 17 (11), 801-815 (2019).</li><li>Surkova, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current+Clinical+Applications+of+Three-Dimensional+Echocardiography:+When+the+Technique+Makes+the+Difference.">Current Clinical Applications of Three-Dimensional Echocardiography: When the Technique Makes the Difference.</a> Current Cardiology Reports. 18 (11), 109 (2016).</li><li>Lang, R. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recommendations+for+cardiac+chamber+quantification+by+echocardiography+in+adults:+an+update+from+the+American+Society+of+Echocardiography+and+the+European+Association+of+Cardiovascular+Imaging.">Recommendations for cardiac chamber quantification by echocardiography in adults: an update from the American Society of Echocardiography and the European Association of Cardiovascular Imaging.</a> Journal of the American Society of Echocardiography. 28 (1), 1-39 (2015).</li><li>Matyas, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+speckle-tracking+echocardiography+with+invasive+hemodynamics+for+the+detection+of+characteristic+cardiac+dysfunction+in+type-1+and+type-2+diabetic+rat+models.">Comparison of speckle-tracking echocardiography with invasive hemodynamics for the detection of characteristic cardiac dysfunction in type-1 and type-2 diabetic rat models.</a> Cardiovascular Diabetology. 17 (1), 13 (2018).</li><li>Kovacs, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impact+of+hemodialysis,+left+ventricular+mass+and+FGF-23+on+myocardial+mechanics+in+end-stage+renal+disease:+a+three-dimensional+speckle+tracking+study.">Impact of hemodialysis, left ventricular mass and FGF-23 on myocardial mechanics in end-stage renal disease: a three-dimensional speckle tracking study.</a> International Journal of Cardiovascular Imaging. 30 (7), 1331-1337 (2014).</li><li>Muraru, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comprehensive+analysis+of+left+ventricular+geometry+and+function+by+three-dimensional+echocardiography+in+healthy+adults.">Comprehensive analysis of left ventricular geometry and function by three-dimensional echocardiography in healthy adults.</a> Journal of the American Society of Echocardiography. 26 (6), 618-628 (2013).</li><li>Lakatos, B. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relationship+between+Cardiac+Remodeling+and+Exercise+Capacity+in+Elite+Athletes:+Incremental+Value+of+Left+Atrial+Morphology+and+Function+Assessed+by+Three-Dimensional+Echocardiography.">Relationship between Cardiac Remodeling and Exercise Capacity in Elite Athletes: Incremental Value of Left Atrial Morphology and Function Assessed by Three-Dimensional Echocardiography.</a> Journal of the American Society of Echocardiography. 33 (1), 101-109 (2020).</li><li>Muraru, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intervendor+Consistency+and+Accuracy+of+Left+Ventricular+Volume+Measurements+Using+Three-Dimensional+Echocardiography.">Intervendor Consistency and Accuracy of Left Ventricular Volume Measurements Using Three-Dimensional Echocardiography.</a> Journal of the American Society of Echocardiography. 31 (2), 158-168 (2018).</li><li>Kalam, K., Otahal, P., Marwick, T. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prognostic+implications+of+global+LV+dysfunction:+a+systematic+review+and+meta-analysis+of+global+longitudinal+strain+and+ejection+fraction.">Prognostic implications of global LV dysfunction: a systematic review and meta-analysis of global longitudinal strain and ejection fraction.</a> Heart. 100 (21), 1673-1680 (2014).</li><li>Muraru, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Validation+of+a+novel+automated+border-detection+algorithm+for+rapid+and+accurate+quantitation+of+left+ventricular+volumes+based+on+three-dimensional+echocardiography.">Validation of a novel automated border-detection algorithm for rapid and accurate quantitation of left ventricular volumes based on three-dimensional echocardiography.</a> European Journal of Echocardiography. 11 (4), 359-368 (2010).</li><li>Doronina, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Female+Athlete's+Heart:+Comparison+of+Cardiac+Changes+Induced+by+Different+Types+of+Exercise+Training+Using+3D+Echocardiography.">The Female Athlete's Heart: Comparison of Cardiac Changes Induced by Different Types of Exercise Training Using 3D Echocardiography.</a> BioMed Research International. 2018, 3561962 (2018).</li><li>Takeuchi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measurement+of+left+ventricular+mass+by+real-time+three-dimensional+echocardiography:+validation+against+magnetic+resonance+and+comparison+with+two-dimensional+and+m-mode+measurements.">Measurement of left ventricular mass by real-time three-dimensional echocardiography: validation against magnetic resonance and comparison with two-dimensional and m-mode measurements.</a> Journal of the American Society of Echocardiography. 21 (9), 1001-1005 (2008).</li><li>Armstrong, A. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=LV+mass+assessed+by+echocardiography+and+CMR,+cardiovascular+outcomes,+and+medical+practice.">LV mass assessed by echocardiography and CMR, cardiovascular outcomes, and medical practice.</a> JACC Cardiovasc Imaging. 5 (8), 837-848 (2012).</li><li>Olah, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+the+dynamic+changes+in+left+ventricular+morphology+and+function+induced+by+exercise+training+and+detraining.">Characterization of the dynamic changes in left ventricular morphology and function induced by exercise training and detraining.</a> International Journal of Cardiology. 277, 178-185 (2019).</li><li>Nagy, V. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+Right+Ventricular+Global+Longitudinal+Strain+in+Predicting+Early+and+Long-Term+Mortality+in+Cardiac+Resynchronization+Therapy+Patients.">Role of Right Ventricular Global Longitudinal Strain in Predicting Early and Long-Term Mortality in Cardiac Resynchronization Therapy Patients.</a> PLoS One. 10 (12), e0143907 (2015).</li><li>Kovacs, A., Lakatos, B., Tokodi, M., Merkely, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Right+ventricular+mechanical+pattern+in+health+and+disease:+beyond+longitudinal+shortening.">Right ventricular mechanical pattern in health and disease: beyond longitudinal shortening.</a> Heart Failure Reviews. 24 (4), 511-520 (2019).</li><li>Badano, L. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+three-dimensional+speckle+tracking+to+assess+left+ventricular+myocardial+mechanics:+inter-vendor+consistency+and+reproducibility+of+strain+measurements.">Use of three-dimensional speckle tracking to assess left ventricular myocardial mechanics: inter-vendor consistency and reproducibility of strain measurements.</a> European Heart Journal - Cardiovascular Imaging. 14 (3), 285-293 (2013).</li></ol>

LV morfologiske og funktionelle målinger repræsenterer hjørnestenene i diagnose, ledelse og opfølgning af hjertesygdomme; desuden er de stærke forudsigere af resultatet. Generelt anbefales 2D-ekkokardiografibaseret evaluering af LV'en i henhold til gældende retningslinjer for praksis. 3D-ekkokardiografi har dog vist sig at være mere præcis, da den er fri for geometriske antagelser vedrørende LV-form7,8. Deformation imaging ved speckle tracking er en robu...

Vurderingen af venstre ventrikulær (LV) morfologi og funktion er det fremherskende formål med generelle og endnu mere specifikke undersøgelser i kardiologi1. Den bredt tilgængelige og ikke-invasive transthoracic ekkokardiografi (TTE), som kan give tætte mængder af information, er den valgte metode til en bekvem, hurtig og omkostningseffektiv evaluering.
Måling af LV masse, volumener, og efterfølgende udslyngning fraktion besidder betydelig diagnostisk og også prognostisk værdi2. Jo mere præcis en given foranstaltning er, jo højere vil dens værdi være. En bedre sammenhæng med guld standard hjerte magnetisk resonans (CMR) imaging afledte værdier er en løbende jagt på ekkokardigrafiske teknikker. Generelt anbefaler retningslinjer for klinisk praksis biplanet Simpsons metode til LV-volumen- og udslyngningsfraktionmåling3. LV er imidlertid en tredimensionel (3D) struktur med en ofte uregelmæssig form, og derfor vil flere tomografiske planer utvivlsomt mislykkes i nogle kliniske scenarier for præcist at afgrænse LV morfologi og funktion. Nylige fremskridt inden for ultralydshardware og softwareteknologi gjorde det muligt at udvikle 3D-billeddannelse i realtid, hvilket revolutionerer ekkokardigrafiske protokoller.
Desuden resulterede behovet for en kvantitativ tilgang vedrørende wall motion abnormiteter i stigningen i deformation imaging4. Strain- og belastningshastighedsparametre kan beregnes ved speckle-sporing ved hjælp af standard gråskalabilleder. 3D-ekkokardiografi kan også overvinde flere mangler ved en todimensionel stammevurdering5. Fra et dyrt videnskabeligt værktøj begyndte 3D-ekkokardiografi at blive en kraftfuld teknik, der blev brugt i daglig klinisk praksis, og kvantificeringen af LV er bestemt i den første linje i dette gennembrud.
Denne artikel har til formål at guide læseren gennem en detaljeret 3D-protokol ved at præsentere tips og tricks og også ved at fremhæve de potentielle faldgruber for at lette den udbredte, men teknisk forsvarlige brug af denne vigtige teknik vedrørende LV.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>3V-D/4V-D/4Vc-D</td>
			<td>General Electric</td>
			<td>n.a.</td>
			<td>ultrasound probe</td>
		</tr>
		<tr>
			<td>4D Auto LVQ</td>
			<td>General Electric</td>
			<td>n.a.</td>
			<td>software for analysis</td>
		</tr>
		<tr>
			<td>E9/E95</td>
			<td>General Electric</td>
			<td>n.a.</td>
			<td>ultrasound machine</td>
		</tr>
		<tr>
			<td>EchoPac v203</td>
			<td>General Electric</td>
			<td>n.a.</td>
			<td>software for analysis</td>
		</tr>
	</tbody>
</table>

evaluation of left ventricular structure and function using 3d echocardiography

Tredimensionel (3D) kvantificering af venstre ventrikel (LV) giver betydelig merværdi med hensyn til diagnostisk nøjagtighed og præcis risiko stratificering i forskellige hjertesygdomme. For nylig blev 3D-ekkokardiografi tilgængelig i rutinemæssig kardiologipraksis; Men, høj kvalitet billede erhvervelse og efterfølgende analyse har en stejl indlæringskurve. Denne artikel har til formål at guide læseren gennem en detaljeret 3D-protokol ved at præsentere tips og tricks og også ved at fremhæve de potentielle faldgruber for at lette den udbredte, men teknisk forsvarlige brug af denne vigtige teknik vedrørende LV. Først og fremmest viser vi erhvervelsen af et 3D-datasæt af høj kvalitet med optimal rumlig og tidsmæssig opløsning. Derefter præsenterer vi de analytiske trin mod en detaljeret kvantificering af LV ved hjælp af en af de mest anvendte indbyggede software. Vi vil kvantificere LV-mængder, sfæriskhed, masse og også systolisk funktion ved at måle udslyngningsfraktion og myokardiedeformation (langsgående og omferentiel stamme). Vi vil diskutere og give kliniske eksempler på de væsentlige scenarier, hvor overgangen fra en konventionel ekkokardiografisk tilgang til en 3D-baseret kvantificering anbefales stærkt.

3D-analyse af LV er mulig hos de fleste patienter. Sag 1 er en sund frivillig med normale ventrikulære volumener og funktion (figur 1). Sag 2 (Figur 2) er en 64-årig mandlig patient med udvidet kardiomyopati og et bredt QRS-kompleks (160 ms) af venstre bundt gren blok morfologi. Cmr-målinger af guldstandard var følgende: end-diastolisk volumen: 243 mL, slutsystolisk volumen: 160 mL, udslyngningsfraktion: 34%, LV-masse: 163 g....

Watch this Scientific Journal Video about Evaluation of Left Ventricular Structure and Function using 3D Echocardiography at JoVE.com

Evaluation of Left Ventricular Structure and Function using 3D Echocardiography

I denne artikel leverer vi en trinvis anskaffelses- og analyseprotokol til volumetrisk vurdering og speckle-tracking-analyse af venstre ventrikel ved 3D-ekkokardiografi, især med fokus på praktiske aspekter, der maksimerer gennemførligheden af denne teknik.

Evaluering af venstre ventrikulær struktur og funktion ved hjælp af 3D-ekkokardiografi

Three-dimensional (3D) quantification of the left ventricle (LV) provides significant added value in terms of diagnostic accuracy and precise risk ...

Research

JoVE Journal

Bioengineering

Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice

Måling af tryk i venstre ventrikel i slutningen af ​​embryoner og neonatale mus

Blood pressure increases significantly during embryonic and postnatal development in vertebrate animals. In the mouse, blood flow is first detectable ...

In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography

In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography

In vivo kvantitativ vurdering af Myokardie Struktur, funktion, Perfusion og levedygtighed Brug Cardiac Micro-computertomografi

The use of Micro-Computed Tomography (MicroCT) for in vivo studies of small animals as models of human disease has risen tremendously due to the fact that ...

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Forbedring 2D og 3D Skin<em&gt; In vitro</em&gt; Modeller Brug Macromolecular Fortrængning

The glycoprotein family of collagens represents the main structural proteins in the human body, and are key components of biomaterials used in modern ...

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

Bioprinting af brusk og huden væv analoger udnytter en roman passiv blanding enhed teknik for Bioink Precellularization

Bioprinting is a powerful technique for the rapid and reproducible fabrication of constructs for tissue engineering applications. In this study, both ...

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Rensning og analyse af et monoklonalt antistof fra ovarieceller fra kinesiske hamster ved hjælp af et automatiseret Mikrobioreaktor system

Monoclonal antibodies (mAbs) are one of the most popular and well-characterized biological products manufactured today. Most commonly produced using ...

Four-Dimensional CT Analysis Using Sequential 3D-3D Registration

Fire dimensional CT-analyse ved hjælp af sekventiel 3D-3D-registrering

Four-dimensional computed tomography (4DCT) provides a series of volume data and visualizes joint motions. However, numerical analysis of 4DCT data ...

Optical Imaging of Isolated Murine Ventricular Myocytes

Optisk billeddannelse af isolerede murine ventrikulære Myocytter

The ability to isolate adult cardiac myocytes has permitted researchers to study a variety of cardiac pathologies at the single cell level. While advances ...

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator

Primær afklaring af CHO Harvested Cell Culture Fluid ved hjælp af en akustisk separator

Primary clarification is an essential step in a biomanufacturing process for the initial removal of cells from therapeutic products within the harvested ...

Measuring 3D In-vivo Shoulder Kinematics using Biplanar Videoradiography

Måling af 3D In-vivo Skulder kinematics ved hjælp af Biplanar Videoradiography

The shoulder is one of the human body's most complex joint systems, with motion occurring through the coordinated actions of four individual joints, ...

Quantification of Mouse Heart Left Ventricular Function, Myocardial Strain, and Hemodynamic Forces by Cardiovascular Magnetic Resonance Imaging

Kvantificering af mus hjerte venstre ventrikulær funktion, myokardiestamme, og hæmodynamiske kræfter ved hjerte-kar-magnetisk resonans imaging

Mouse models have contributed significantly to understanding genetic and physiological factors involved in healthy cardiac function, how perturbations ...