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DOI :
10.3791/61224-v
•
11:53 min
June 14th, 2020
Chapters
0:04
Introduction
1:29
Surgical Procedure
3:30
Atrial Cell Isolation and Culture
5:46
Ventricular Cell Isolation and Culture
7:46
Stepwise Paradigm Calcium Reintroduction
9:04
Results: Representative Adult Mouse Heart Atrial and Ventricular Myocyte and Non-Myocyte Characterization
11:09
Conclusion
一种方法被描述为同时从单个成年小鼠心脏的腹膜和心室中分离出肌细胞和非肌细胞。此协议可产生高度可行的心肌细胞和非肌细胞的一致产量,并详细说明表型和体外分析的最佳细胞特定培养条件。
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