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DOI :
10.3791/61224-v
•
11:53 min
June 14th, 2020
Chapters
0:04
Introduction
1:29
Surgical Procedure
3:30
Atrial Cell Isolation and Culture
5:46
Ventricular Cell Isolation and Culture
7:46
Stepwise Paradigm Calcium Reintroduction
9:04
Results: Representative Adult Mouse Heart Atrial and Ventricular Myocyte and Non-Myocyte Characterization
11:09
Conclusion
単成マウス心臓の心房と心室の両方から筋細胞と非筋細胞を同時に分離する方法について説明する。このプロトコルは、高生存性心筋細胞および非筋細胞の一貫した収量をもたらし、フェノタイピングおよびインビトロ分析のための最適な細胞特異的培養条件を詳述する。
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