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DOI :
10.3791/61225-v
June 10th, 2020
Chapters
0:04
Introduction
0:42
96-Well Plate Coating
2:49
Induced Pluripotent Stem Cell (iPSC) Culture Maintenance
5:03
Cell Confluence Characterization
6:53
Cytoskeletal Microfilament Characterization
9:02
Results: Representative Analyses of Cell Confluence on Differently Coated Vessels
10:57
Conclusion
このプロトコルの目的は、さまざまな細胞外マトリックス(ECM)コーティング条件を比較して、差動コーティングが人工多能性幹細胞(iPSC)の増殖速度にどのように影響するかを評価することです。特に、iPS細胞培養の最適な増殖を得るための条件設定を目指しています。
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