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DOI :
10.3791/61225-v
June 10th, 2020
Chapters
0:04
Introduction
0:42
96-Well Plate Coating
2:49
Induced Pluripotent Stem Cell (iPSC) Culture Maintenance
5:03
Cell Confluence Characterization
6:53
Cytoskeletal Microfilament Characterization
9:02
Results: Representative Analyses of Cell Confluence on Differently Coated Vessels
10:57
Conclusion
이 프로토콜의 목표는 다양한 세포외 기질(ECM) 코팅 조건을 비교하여 차등 코팅이 유도만능줄기세포(iPSC)의 성장 속도에 어떤 영향을 미치는지 평가하는 것입니다. 특히, 우리는 iPSC 배양의 최적 성장을 얻기위한 조건을 설정하는 것을 목표로합니다.
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