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DOI :
10.3791/61282-v
September 9th, 2020
Chapters
0:05
Introduction
1:13
Craniotomy and Animal Preparation
2:16
AAV Cocktail Injection
3:08
In Vivo Neural Recordings with Optogenetics
5:20
Amplifier and Filter Settings
7:23
Results: VTA Glutamate Projections to the Hippocampus Modulate CA1 Activity
8:42
Conclusion
現在のプロトコルは、海馬への腹側テグメンタル領域(VTA)グルタミン酸突起をトレースするための簡単な方法を示しています。VTAグルタミン酸ニューロンの光刺激をCA1記録と組み合わせて、VTAグルタミン酸末端が生体内での推定ピラミッド発火率を調節する方法を示した。
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