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DOI :
10.3791/61285-v
June 13th, 2020
Chapters
0:04
Introduction
0:34
Bacterial Cell Culture and Lactobacillus Cell-Free Supernatant (LCFS) Preparation
2:18
Colorectal Cancer Cell Line Preparation
3:12
Spheroid Formation
3:49
3D colorectal Cancer Cell LCFS Treatment
4:26
Results: Representative Effects of LCFS Co-Culture on Spheroid Formation and Viability
5:22
Conclusion
这里介绍了了解 乳酸杆菌 无细胞超高分子(LCFS)的抗癌作用的方法。结肠直肠癌细胞系显示在3D培养物中用LCFS治疗时细胞死亡。生成球体的过程可以根据脚手架进行优化,并且所呈现的分析方法可用于评估所涉及的信号通路。
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